Tissue factor Antikörper (AA 32-100)

Produktnummer ABIN708086

Produktdetails anti-Tissue factor Antikörper

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Bindungsspezifität: AA 32-100

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tissue factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Schwein, Ratte

Homologie: Dog,Cow,Horse,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD142

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #029589 (Immunohistochemistry)

by: Reveal Biosciences

No.: #029589

Datum: 07.02.2014

Chargennummer: 980886W

Validierte Anwendung: Immunohistochemistry

Positivkontrolle: Mouse lung tissue

Negativkontrolle: Mouse brain tissue

Bewertung: Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.

Validierungsbilder

Figure 1: Mouse lung tissue stained with anti-F3 (brown) and counterstained in blue.

Figure 2: Mouse brain tissue stained with anti-F3 (brown) and counterstained in blue.

Figure 3: Mouse lung tissue stained with isotype control antibody (brown) and counterstained in blue.

Figure 4: Mouse lung tissue stained with secondary antibody only (brown) and counterstained in blue.

Protokoll

Primärantikörper:

• Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
• Catalog number: ABIN708086
• Lot number: 120224

Sekundärantikörper:

• Antibody: Bond Polymer Refine Detection Kit
• Lot number: 24144

Full Protocol:

• Immunohistochemistry was performed on a Leica Bond automated immunostainer.
• Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
• Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
• Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
• Sections were washed x 3 in Leica Bond Wash Buffer.
• Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
• Sections were washed x 3 in Leica Bond Wash Buffer.
• Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
• Sections were washed x 4 in Leica Bond Wash Buffer.
• Sections were washed x 1 in Distilled Water.
• Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
• Sections were washed x 4 in Leica Bond Wash Buffer.
• Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
• Sections were washed x 3 in Distilled Water.
• Sections were counterstained with hematoxylin (Leica) for 2 minutes.
• Sections were washed x 1 in Distilled Water.
• Sections were washed x 1 in Leica Bond Wash Buffer.
• Sections were washed x 1 in Distilled Water.
• Sections were dehydrated, mounted and photographed under a light microscope.

Anmerkungen: None notes.

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Tissue factor Antikörper (ABIN708086)

Fletcher, Das, Gadye, Street, Baudhuin, Wagner, Cole, Flores, Choi, Yosef, Purdom, Dudoit, Risso, Ngai: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).

Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).

Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).

Gleeson, Martin, Ali, Kumar, Pillai, Kumar, OSullivan, Whelan, Stocca, Khider, Barry, OBrien, Caplice: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), (2015) (PubMed).

Tian, Salsbery, Wang, Yuan, Yang, Zhao, Wu, Zhang, Konkle, Thiagarajan, Li, Zhang, Dong: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, (2015) (PubMed).

Milano, Dongiovanni, Artoni, Gatti, Rosso, Colombo, Bollati, Maggioni, Mannucci, Bertazzi, Fargion, Valenti: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, (2015) (PubMed).

Liu, Wei, Zhang, Xu, Wang, Zhou: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).

Details zu Tissue factor

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Andere Bezeichnung: CD142 (F3 Produkte)

Hintergrund:

Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Gen-ID: 2152

UniProt: P13726

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling