Phospho ERK1/2 Flex Set (Bead C4)

Produktnummer ABIN1379692

Produktdetails

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Bindungsspezifität: pThr202, pTyr204

Reaktivität: Human, Maus, Ratte

Applikation: Immunoassay (IA), Flow Cytometry (FACS)

Marke: BD™ Cytometric Bead Array,BD™

Produktmerkmale: The BD™ CBA Phospho ERK1/2 (T202/Y204) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat extracellular signal regulated kinases 1/2 (ERK1/2), a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-202 and tyrosine-phosphorylated at Tyr-204 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho ERK1/2 (T202/Y204) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho ERK1/2 (T202/Y204) Flex Set. The biology and function of ERK1/2 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.
The Phospho ERK1/2 (T202/Y204) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

Bestandteile: Capture beads, detection reagent, standards

Anwendungsinformationen

Kommentare:

Bead Position: C4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho ERK1/2 (T202/Y204) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

Referenzen

Boldt, Kolch: "Targeting MAPK signalling: Prometheus' fire or Pandora's box?" in: Current pharmaceutical design, Vol. 10, Issue 16, pp. 1885-905, (2004) (PubMed).

Antigendetails

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Andere Bezeichnung: ERK1/2 (MAPK1/3 Produkte)

Synonyme: erk1/2 Accessory Reagents, mitogen-activated protein kinase Accessory Reagents, erk1/2 Accessory Reagents