Maus TrueBlot® Anti-Maus Ig Biotin

Produktnummer ABIN1589968

Produktdetails

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Immunoprecipitation (IP), Western Blotting (WB)

Marke: TrueBlot®

Produktmerkmale: Mouse IgG TrueBlot® ULTRA is a unique anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blotting with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. The Biotin Mouse TrueBlot® ULTRA is the biotinylated format of Mouse IgG TrueBlot® ULTRA. It can be used as a secondary antibody, followed by the use of avidin-peroxidase conjugated (A003-03).
Conjugation Name: Biotin TrueBlot® ULTRA

Bestandteile: Mouse TrueBlot®: Anti-Mouse Ig Biotin

Anwendungsinformationen

Applikationshinweise: Western Blot Dilution: 1:2000

Kommentare:

Biotin Mouse TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Biotin Mouse TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Biotin Mouse TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Note: There are two key procedural considerations:
1. When using any TrueBlot® reagent, ensure there is complete reduction of the lysate.
2. Use milk powder for complete and effective blocking of the western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: Proprietary

0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze. Dilute only prior to immediate use.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.

Haltbarkeit: 6 months

Referenzen

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Zhang, Ozawa, Lee, Wen, Tan, Wadzinski, Seto: "Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4." in: Genes & development, Vol. 19, Issue 7, pp. 827-39, (2005) (PubMed).

DiPerna, Stack, Bowie, Boyd, Kotwal, Zhang, Arvikar, Latz, Fitzgerald, Marshall: "Poxvirus protein N1L targets the I-kappaB kinase complex, inhibits signaling to NF-kappaB by the tumor necrosis factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by toll-like receptors." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36570-8, (2004) (PubMed).

Lehtonen, Lehtonen, Kudlicka, Holthöfer, Farquhar: "Nephrin forms a complex with adherens junction proteins and CASK in podocytes and in Madin-Darby canine kidney cells expressing nephrin." in: The American journal of pathology, Vol. 165, Issue 3, pp. 923-36, (2004) (PubMed).

Tyagi, Agarwal, Harrison, Glode, Agarwal: "Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages." in: Carcinogenesis, Vol. 25, Issue 9, pp. 1711-20, (2004) (PubMed).