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TrueBlot® Anti-Rabbit IgG Magnetic Beads

Produktnummer ABIN6809831
  • Applikation
    Immunoprecipitation (IP), Western Blotting (WB)
    Verwendungszweck
    TrueBlot® goat Anti-rabbit IgG magnetic beads can be used for separation and purification of rabbit antibodies from serum or rabbit antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots.
    Marke
    TrueBlot®
    Produktmerkmale
    TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as goat Anti-rabbit IgG, and are specifically designed, tested and quality controlled for isolation and purification of rabbit IgG, and immunoprecipitation methods using manual or automatic platforms. This antibody binds the heavy chain of all rabbit IgG subclasses and is suitable for immunoassays that utilize a rabbit IgG primary polyclonal antibody. Cell separation and sorting can be achieved using a rabbit IgG antibody to defined cell surface antigens. The beads have a large surface area with high capture efficiencies. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL.
  • Applikationshinweise
    Western Blot : User Optimized
    ImmunoPrecipitation: User Optimized
    Other Dilution: User Optimized
    Kommentare

    TrueBlot® goat Anti-rabbit IgG magnetic beads can be used for separation and purification of rabbit antibodies from serum or rabbit antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots. For antibody purification, goat Anti-rabbit IgG magnetic beads are incubated with the rabbit antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually. For IP, target specific antibody is incubated with goat Anti-rabbit IgG magnetic beads. The unbound antibody is washed and the sample containing target antigen is added. After unbound particulates are washed from the beads, the purified protein is eluted from the beads using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Stabilizer: None
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to opening. DO NOT FREEZE.
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