CUT&RUN Concanavalin A Beads

Produktnummer ABIN6923139

Produktdetails

Applikation: Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Separation (Sep)

Verwendungszweck: CUT&RUN Concanavalin A Beads of our CUT&RUN Sets.

Spezifität: The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate.

Produktmerkmale: Concanavalin A is covalently attached to functionalized Magnetic iron oxide particles for use in glycoprotein isolations from serum and cellular extracts.

Bestandteile: Concanavalin A coated particles

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

• Metal ions (calcium and manganese) mediate the binding to Concanavalin A and stabilize is conformation.
• The use of buffers with EDTA or other metal chelators must be avoided as it will result in a loss of carbohydrate binding ability.

Aufbereitung der Reagenzien:

Prepare CUT&RUN Concanavalin A Beads

• Pipette 10 µL CUT&RUN Concanavalin A Beads slurry for each sample into a tube
• Place the tubes on a magnet separator and remove the liquid carefully
• Remove the tubes from the magnetic separator
• Wash beads 3 more times with 1 mL Binding Buffer
• Finally resuspend the beads with 10 µL Binding Buffer per sample

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 5 μg/μL

Buffer: Stored in 10 mM PBS with 0.1 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze the product!
Vortex bead suspension well before use.

Lagerung: 4 °C