AGO10 Antikörper
Dieses Anti-AGO10-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von AGO10 in WB. Geeignet für Arabidopsis thaliana.
Kurzübersicht für AGO10 Antikörper (ABIN4966095)
Target
AGO10 (ZLL)
(eIF2C Family Protein Argonaute 10 (ZLL))
Reaktivität
Arabidopsis thaliana
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
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Kreuzreaktivität (Details)
- Not reactive in: Zea mays
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Homologie
- A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii
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Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810
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Applikationshinweise
- 1: 10 000 (WB)
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Kommentare
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AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Rekonstitution
- For reconstitution add 50 μL of sterile water.
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Informationen zur Lagerung
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- AGO10 (ZLL) (eIF2C Family Protein Argonaute 10 (ZLL))
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Andere Bezeichnung
- Argonaute 10 (AGO10)
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Hintergrund
- AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS).
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Molekulargewicht
- 110.9 kDa
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UniProt
- Q9XGW1
Target
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