ANKRD30B Protein (AA 1-1392) (Strep Tag)
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- Target
- ANKRD30B (Ankyrin Repeat Domain 30B (ANKRD30B))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-1392
- Spezies
- Human
- Quelle
- Tobacco (Nicotiana tabacum)
- Aufreinigungstag / Konjugat
- Dieses ANKRD30B Protein ist gelabelt mit Strep Tag.
- Applikation
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequenz
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MKRLLAAAGK GVRGPEPPNP FSERVYTEKD YGTIYFGDLG KIHTAASRGQ VQKLEKMTVG KKPVNLNKRD MKKRTALHWA CVNGHAEVVT FLVDRKCQLN VLDGEGRTPL MKALQCEREA CANILIDAGA DLNYVDVYGN TALHYAVYSE NLLMVATLLS YGAVIEVQNK ASLTPLLLAI QKRSKQTVEF LLTKNANANA FNESKCTALM LAICEGSSEI VGMLLQQNVD VFAEDIHGIT AERYAAACGV NYIHQQLLEH IRKLPKNPQN TNPEGTSTGT PDEAAPLAER TPDTAESLLE KTPDEAARLV EGTSAKIQCL GKATSGKFEQ STEETPRKIL RPTKETSEKF SWPAKERSRK ITWEEKETSV KTECVAGVTP NKTEVLEKGT SNMIACPTKE TSTKASTNVD VSSVEPIFSL FGTRTIENSQ CTKVEEDFNL ATKIISKSAA QNYTCLPDAT YQKDIKTINH KIEDQMFPSE SKREEDEEYS WDSGSLFESS AKTQVCIPES MYQKVMEINR EVEELPEKPS AFKPAVEMQK TVPNKAFELK NEQTLRAAQM FPSESKQKDD EENSWDSESP CETVSQKDVY LPKATHQKEF DTLSGKLEES PVKDGLLKPT CGRKVSLPNK ALELKDRETF KAESPDKDGL LKPTCGRKVS LPNKALELKD RETLKAESPD NDGLLKPTCG RKVSLPNKAL ELKDRETFKA AQMFPSESKQ KDDEENSWDF ESFLETLLQN DVCLPKATHQ KEFDTLSGKL EESPDKDGLL KPTCGMKISL PNKALELKDR ETFKAEDVSS VESTFSLFGK PTTENSQSTK VEEDFNLTTK EGATKTVTGQ QERDIGIIER APQDQTNKMP TSELGRKEDT KSTSDSEIIS VSDTQNYECL PEATYQKEIK TTNGKIEESP EKPSHFEPAT EMQNSVPNKG LEWKNKQTLR ADSTTLSKIL DALPSCERGR ELKKDNCEQI TAKMEQTKNK FCVLQKELSE AKEIKSQLEN QKAKWEQELC SVRLTLNQEE EKRRNVDILK EKIRPEEQLR KKLEVKQQLE QTLRIQDIEL KSVTSNLNQV SHTHESENDL FHENCMLKKE IAMLKLEVAT LKHQHQVKEN KYFEDIKILQ EKNAELQMTL KLKQKTVTKR ASQYREQLKV LTAENTMLTS KLKEKQDKEI LETEIESHHP RLASALQDHD QSVTSRKNQE LAFHSAGDAP LQGIMNVDVS NTIYNNEVLH QPLYEAQRKS KSPKINLNYA GDDLRENALV SEHAQRDRCE TQCQMKKAEH MYQNEQDNVD KHTEQQESLE QKLFQLESKN RWLRQQLVYA HKKVNKSKVT INIQFPEMKM QRHLNEKNEE VFNYGNHLKE RIDQYEKEKA EREVSIKKYK YFSNFLKESG LG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Produktmerkmale
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Aufreinigung
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Reinheit
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ANKRD30B (Ankyrin Repeat Domain 30B (ANKRD30B))
- Andere Bezeichnung
- ANKRD30B
- Synonyme
- NY-BR-1.1 Protein, ankyrin repeat domain 30B Protein, ANKRD30B Protein
- Hintergrund
- Ankyrin repeat domain-containing protein 30B (Serologically defined breast cancer antigen NY-BR-1.1)
- Molekulargewicht
- 158.0 kDa
- UniProt
- Q9BXX2
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