GFP Antikörper (FITC)

Produktnummer ABIN100088

Hersteller Produkt- Nr.: 600-102-215

Produktdetails anti-GFP Antikörper (FITC)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Hersteller Produkt- Nr.: 600-102-215

Hersteller: Rockland

Verwendungszweck: GFP Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Produktmerkmale: Synonyms: goat anti-GFP Antibody fluorescein Conjugation, FITC conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Aufreinigung: GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Labeling Ratio: 3.85

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-GFP Fluorescein Conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GFP Antikörper (FITC) (ABIN100088)

Perna, Alberi: "TF-ChIP Method for Tissue-Specific Gene Targets." in: Frontiers in cellular neuroscience, Vol. 13, pp. 95, (2019) (PubMed).

Hock, Maniecka, Hruska-Plochan, Reber, Laferrière, Sahadevan M K, Ederle, Gittings, Pelkmans, Dupuis, Lashley, Ruepp, Dormann, Polymenidou: "Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function." in: Cell reports, Vol. 24, Issue 4, pp. 987-1000.e7, (2018) (PubMed).

Kwon, Everetts, Wang, Wang, Della Croce, Xing, Yao: "Controlling Depth of Cellular Quiescence by an Rb-E2F Network Switch." in: Cell reports, Vol. 20, Issue 13, pp. 3223-3235, (2018) (PubMed).

Fong, Mazo, Das, Goodman, Kim, ORourke, Izquierdo, Tsou: "53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis." in: eLife, Vol. 5, (2016) (PubMed).

Doucet, Woo, Ruiz, Owens: "The touch dome defines an epidermal niche specialized for mechanosensory signaling." in: Cell reports, Vol. 3, Issue 6, pp. 1759-65, (2014) (PubMed).

Wang, Tay, Soni, Perumal, Goll, Macaluso, Asara, Amack, Tsou: "CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base." in: Nature cell biology, Vol. 15, Issue 6, pp. 591-601, (2013) (PubMed).

Guo, Maeda, Ma, Delgado, Sohn, Miers, Ko, Bannerman, Xu, Wang, Zhou, Takebayashi, Pleasure: "Macroglial plasticity and the origins of reactive astroglia in experimental autoimmune encephalomyelitis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 33, pp. 11914-28, (2011) (PubMed).

Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." in: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP (GFP Produkte)

Hintergrund: Background: GFP Fluorescein Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P42212