HA-Tag Antikörper

Produktnummer ABIN100176

Produktdetails anti-HA-Tag Antikörper

Target: HA-Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HA-Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Sequenz: YPYDVPDYA

Spezifität: This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

Produktmerkmale: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of

Sterilität: Sterile filtered

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.95 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-HA-Tag Antikörper (ABIN100176)

Wallis, Ventimiglia, Otigbah, Infante, Cuesta-Geijo, Kidiyoor, Carbajal, Fleck, Foiani, Garcia-Manyes, Martin-Serrano, Agromayor: "The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair." in: Developmental cell, Vol. 56, Issue 23, pp. 3192-3202.e8, (2021) (PubMed).

Merigliano, Burla, La Torre, Del Giudice, Teo, Liew, Chojnowski, Goh, Olmos, Maccaroni, Giubettini, Chiolo, Carlton, Raimondo, Vernì, Stewart, Rhodes, Wright, Burke, Saggio: "AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody." in: PLoS genetics, Vol. 17, Issue 8, pp. e1009757, (2021) (PubMed).

Alvarez-Castelao, Tom Dieck, Fusco, Donlin-Asp, Perez, Schuman: "The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition." in: eLife, Vol. 9, (2020) (PubMed).

Nuwer, Fleck: "Anterograde trafficking signals in GABAA subunits are required for functional expression." in: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).

Rohde, Becker, Krähling: "Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication." in: Emerging microbes & infections, Vol. 8, Issue 1, pp. 1300-1313, (2020) (PubMed).

Stedden, Menegas, Zajac, Williams, Cheng, Özkan, Horne-Badovinac: "Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila." in: Current biology : CB, Vol. 29, Issue 6, pp. 908-920.e6, (2020) (PubMed).

Pati, Salvi, Kallianpur, Vaidya, Banerjee, Maiti, Clement, Vaidya: "Chemogenetic Activation of Excitatory Neurons Alters Hippocampal Neurotransmission in a Dose-Dependent Manner." in: eNeuro, Vol. 6, Issue 6, (2020) (PubMed).

Cousineau, Lescouzères, Taupignon, Delgado-Zabalza, Valjent, Baufreton, Le Bon-Jégo: "Dopamine D2-Like Receptors Modulate Intrinsic Properties and Synaptic Transmission of Parvalbumin Interneurons in the Mouse Primary Motor Cortex." in: eNeuro, Vol. 7, Issue 3, (2020) (PubMed).

Ventimiglia, Cuesta-Geijo, Martinelli, Caballe, Macheboeuf, Miguet, Parnham, Olmos, Carlton, Weissenhorn, Martin-Serrano: "CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope." in: Developmental cell, Vol. 47, Issue 5, pp. 547-563.e6, (2019) (PubMed).

Yamada, Murata, Adachi, Itoh, Kameoka, Igarashi, Kato, Araki, Huganir, Dawson, Yanagawa, Okamoto, Iijima, Sesaki: "Mitochondrial Stasis Reveals p62-Mediated Ubiquitination in Parkin-Independent Mitophagy and Mitigates Nonalcoholic Fatty Liver Disease." in: Cell metabolism, Vol. 28, Issue 4, pp. 588-604.e5, (2019) (PubMed).

Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Kijima, Prince, Tigue, Yim, Schwartz, Beebe, Lee, Budzynski, Williams, Trepel, Sistonen, Calderwood, Neckers: "HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation." in: Scientific reports, Vol. 8, Issue 1, pp. 6976, (2018) (PubMed).

Ulu, Oh, Zuo, Frost: "Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion." in: Journal of cell science, Vol. 131, Issue 3, (2018) (PubMed).

Bohn, Thummar, York, Raymond, Brown, Bieniasz, Hatziioannou, Smith: "APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA." in: Nature communications, Vol. 8, Issue 1, pp. 1021, (2018) (PubMed).

Yue, Nabar, Shi, Kamenyeva, Xiao, Hwang, Wang, Kehrl: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." in: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).

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York, Kutluay, Errando, Bieniasz: "The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid." in: PLoS pathogens, Vol. 12, Issue 8, pp. e1005833, (2017) (PubMed).

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Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

Details zu HA-Tag

Target: HA-Tag

Andere Bezeichnung: HA tag (HA-Tag Produkte)

Substanzklasse: Tag

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of