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Hub1 Antikörper

Hub1 Reaktivität: Saccharomyces cerevisiae WB, ELISA, IP, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN100576
  • Target
    Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
    Reaktivität
    Saccharomyces cerevisiae
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.
    Immunogentype:Recombinant
    Isotyp
    IgG
  • Applikationshinweise
    This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Restore with deionized water (or equivalent)
    Konzentration
    5.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
    Andere Bezeichnung
    Hub1
    Hintergrund
    Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Hub1 (''Homologous to UBiquitin'') may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation.
    Synonyms: Homologous to UBiquitin antibody, Hub1 protein antibody, HUB1 target protein 1 antibody, Hub1p antibody, Ubiquitin like protein modifier antibody
    Gen-ID
    855767, 6324360
    UniProt
    Q6Q546
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