KLH Antikörper

Produktnummer ABIN103377

Produktdetails anti-KLH Antikörper

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reaktivität: Keyhole Limpet

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLH Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Sterilität: Sterile filtered

Immunogen: KLH (Keyhole Limpet Hemocyanin)
Immunogentype:Native

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-KLH Antikörper (ABIN103377)

Datta, Silverman, Phipps, Hiraragi, Ratner, Lairmore: "Human T-lymphotropic virus type-1 p30 alters cell cycle G2 regulation of T lymphocytes to enhance cell survival." in: Retrovirology, Vol. 4, pp. 49, (2007) (PubMed).

Details zu KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Abstract: KLH Produkte

Hintergrund: KLH Antibody detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: Keyhole Limpet Hemocyanin, KLH, Anti-KLH antibody