KLH Antikörper (Biotin)

Produktnummer ABIN103379

Produktdetails anti-KLH Antikörper (Biotin)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reaktivität: Keyhole Limpet

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLH Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: KLH (Keyhole Limpet Hemocyanin
Immunogentype:Native

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Anti-KLH antibody biotin conjugate is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Abstract: KLH Produkte

Hintergrund: KLH Antibody detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: Keyhole Limpet Hemocyanin