Anti-KLH Antibody Peroxidase Conjugated antibody has been assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 is suggested for this product. Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Restore with deionized water (or equivalent)
Konzentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer. Synonyms: HRP, Keyhole Limpet Hemocyanin