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KLH Antikörper (HRP)

KLH Reaktivität: Keyhole Limpet ELISA, WB Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN103381
  • Target Alle KLH Antikörper anzeigen
    KLH (Keyhole Limpet Hemocyanin (KLH))
    Reaktivität
    • 40
    • 11
    • 3
    • 1
    Keyhole Limpet
    Wirt
    • 25
    • 18
    • 8
    • 4
    Kaninchen
    Klonalität
    • 41
    • 10
    Polyklonal
    Konjugat
    • 16
    • 9
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser KLH Antikörper ist konjugiert mit HRP
    Applikation
    • 27
    • 25
    • 13
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    KLH (Keyhole Limpet Hemocyanin
    Immunogentype:Native
    Isotyp
    IgG
    Top Product
    Discover our top product KLH Primärantikörper
  • Applikationshinweise
    Anti-KLH Antibody Peroxidase Conjugated antibody has been assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 is suggested for this product.  Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Restore with deionized water (or equivalent)
    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Konservierungsmittel
    Gentamicin sulfate
    Handhabung
    Do NOT add Sodium Azide!
    Lagerung
    4 °C
  • Target
    KLH (Keyhole Limpet Hemocyanin (KLH))
    Abstract
    KLH Produkte
    Hintergrund
    KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
    Synonyms: HRP, Keyhole Limpet Hemocyanin
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