Left-Right Determination Factor (LEFTY-A) Antikörper

Produktnummer ABIN103459

Produktdetails

Target: Left-Right Determination Factor (LEFTY-A)

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Sterilität: Sterile filtered

Immunogen: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Immunogentype:Recombinant

Klon: 7C5G1H6H10

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for ELISA and western blotting.  The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen

Ulloa, Tabibzadeh: "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21397-404, (2001) (PubMed).

Tsukui, Capdevila, Tamura, Ruiz-Lozano, Rodriguez-Esteban, Yonei-Tamura, Magallón, Chandraratna, Chien, Blumberg, Evans, Belmonte: "Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11376-81, (1999) (PubMed).

Meno, Shimono, Saijoh, Yashiro, Mochida, Ohishi, Noji, Kondoh, Hamada: "lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal." in: Cell, Vol. 94, Issue 3, pp. 287-97, (1998) (PubMed).

Antigendetails

Target: Left-Right Determination Factor (LEFTY-A)

Andere Bezeichnung: LEFTY A (LEFTY-A Produkte)

Hintergrund: During vertebrate embryogenesis, a left-right axis is established.  Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries.  TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors.   Complexes formed move into the nucleus, where they act as components of a transcriptional complex.  Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling.  Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor.  Lefty also inhibits events downstream from R-Smad phosphorylation.  Lefty provides a repressed state of TGF-beta-responsive genes.  The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.  Members of the TGF-beta superfamily require processing for their activation.   Cleavage is therefore an essential step for Lefty activation.  Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
Synonyms: Left-right determination factor 2 antibody, Protein lefty-2 antibody, Left-right determination factor A antibody, Protein lefty-A antibody, Transforming growth factor beta-4 antibody, TGF-beta-4 antibody,Endometrial bleeding-associated factor antibody

Gen-ID: 320202

UniProt: P57785, O00292