alpha 2 Macroglobulin Antikörper (Biotin)

Produktnummer ABIN103763

Produktdetails anti-alpha 2 Macroglobulin Antikörper (Biotin)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser alpha 2 Macroglobulin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: Alpha-2-Macroglobulin Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum as well as purified and partially purified alpha-2-MACROGLOBULIN [Human Plasma].

Produktmerkmale: Synonyms: goat anti-Alpha-2-Macroglobulin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

Aufreinigung: Anti-Alpha-2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: alpha-2-MACROGLOBULIN [Human Plasma]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Alpha-2-Macroglobulin has been tested by western blot and is suitable to be assayed against 1.0 μg of alpha-2-MACROGLOBULIN in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Andere Bezeichnung: A2M (A2M Produkte)

Hintergrund: Background: Anti-alpha-2-Macroglobulin antibody is secreted into the plasma and belongs to the protease inhibitor I39 family. Anti-alpha-2-Macroglobulin inhibits all proteinases utilizing a ""bait region,"" or specific cleavage site, which traps the proteinase, after which a thioester bond is hydrolyzed and regulates the covalent binding of the protein to proteinase. Anti-alpha-2-Macroglobulin antibody is ideal for investigators interested in Cardiovascular , Protease Inhibitor, or Cell Biology research.

Gen-ID: 2

NCBI Accession: NP_000005

UniProt: P01023

Pathways: Lipid Metabolism