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Malate Dehydrogenase (MDH) Antikörper

MDH Reaktivität: Schwein ELISA, WB, IP Wirt: Schaf Polyclonal unconjugated
Produktnummer ABIN103904
  • Target Alle Malate Dehydrogenase (MDH) Antikörper anzeigen
    Malate Dehydrogenase (MDH)
    Reaktivität
    • 10
    • 4
    Schwein
    Wirt
    • 10
    • 4
    Schaf
    Klonalität
    • 14
    Polyklonal
    Konjugat
    • 7
    • 5
    • 2
    Unkonjugiert
    Applikation
    • 14
    • 14
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    Kreuzreaktivität (Details)
    Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
    Reinheit
    Anti-MALATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].
    Endotoxin-Niveau
    Low Endotoxin : No
    Immunogen
    Malate Dehydrogenase [Pig Heart]
    Immunogen Type: Native Protein
    Isotyp
    IgG
  • Applikationshinweise
    MALATE DEHYDROGENASE antibody has been assayed against 1.0 µg of Malate Dehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:40.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Handhabung
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Haltbarkeit
    Expiration date is one (1) year from date of opening.
  • Target
    Malate Dehydrogenase (MDH)
    Andere Bezeichnung
    Malate Dehydrogenase (MDH Produkte)
    Synonyme
    CG5889 antikoerper, Dmel\\CG5889 antikoerper, MDH antikoerper, Mdh antikoerper, anon-EST:Posey79 antikoerper, MALATE DEHYDROGENASE antikoerper, malate dehydrogenase antikoerper, An15g00070 antikoerper, AO090701000013 antikoerper, DDBDRAFT_0188779 antikoerper, DDBDRAFT_0230186 antikoerper, DDB_0188779 antikoerper, DDB_0230186 antikoerper, Malic enzyme b antikoerper, oxidoreductase, putative antikoerper, malate dehydrogenase antikoerper, NAD dependent malate dehydrogenase Mdh antikoerper, Men-b antikoerper, malate dehydrogenase antikoerper, MDH antikoerper, mdh antikoerper, APH_RS02635 antikoerper, ANI_1_12134 antikoerper, AOR_1_26114 antikoerper, PMI_RS16925 antikoerper, mdhA antikoerper, mdh1 antikoerper
    Hintergrund
    Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase
    Gen-ID
    396894
    UniProt
    P11708
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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