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Merlin Antikörper (pSer518)

Rockland 600-401-414 NF2 Reaktivität: Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN104130
Hersteller Produkt- Nr.: 600-401-414
  • Target Alle Merlin (NF2) Antikörper anzeigen
    Merlin (NF2) (Neurofibromin 2 (NF2))
    Bindungsspezifität
    • 27
    • 15
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer518
    Reaktivität
    • 68
    • 57
    • 45
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Maus
    Wirt
    • 79
    • 4
    • 1
    Kaninchen
    Klonalität
    • 81
    • 3
    Polyklonal
    Konjugat
    • 45
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Merlin Antikörper ist unkonjugiert
    Applikation
    • 69
    • 29
    • 27
    • 26
    • 18
    • 16
    • 11
    • 10
    • 6
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    600-401-414
    Hersteller
    Rockland
    Verwendungszweck
    NF2 phospho S518 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product).
    Produktmerkmale
    Synonyms: rabbit anti-NF2 pS518 antibody, rabbit anti-Merlin pS518 antibody, moesin ezrin radixin like protein antibody, Neurofibromatosis 2 antibody, Neurofibromin-2, Schwannomerlin, Schwannomin, Merlin, NF 2, NF-2, SCH
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
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  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA.  By ELISA, the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Immunoblotting will detect human and mouse NF2 (Merlin).  Although not tested, this antibody is likely functional in Immunofluorescence, immunohistochemistry, and immunoprecipitation.

    Western Blot Dilution: 1:500- 1:2,000

    ELISA Dilution: 1:1,200,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Hu, Du, Shelton, Oldham, DiPersio, Klein: "An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice." in: Cell stem cell, Vol. 21, Issue 1, pp. 91-106.e6, (2018) (PubMed).

    Angelo, Wu, Meng, Sun, Kopetz, McCutcheon, Slopis, Kurzrock: "Combining Curcumin (Diferuloylmethane) and Heat Shock Protein Inhibition for Neurofibromatosis 2 Treatment: Analysis of Response and Resistance Pathways." in: Molecular cancer therapeutics, (2011) (PubMed).

    Lallemand, Saint-Amaux, Giovannini: "Tumor-suppression functions of merlin are independent of its role as an organizer of the actin cytoskeleton in Schwann cells." in: Journal of cell science, Vol. 122, Issue Pt 22, pp. 4141-9, (2010) (PubMed).

    Wang, Lu, Tang, Wang, Wu: "The phosphorylation status of merlin in sporadic vestibular Schwannomas." in: Molecular and cellular biochemistry, Vol. 324, Issue 1-2, pp. 201-6, (2009) (PubMed).

  • Target
    Merlin (NF2) (Neurofibromin 2 (NF2))
    Andere Bezeichnung
    NF2 (NF2 Produkte)
    Hintergrund
    Background: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin).  The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERM's suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane.  The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75 kDa.  This antibody is specific for the phosphorylated form of human NF2 (Merlin).
    Gen-ID
    4771
    NCBI Accession
    NP_000259
    UniProt
    P35240
    Pathways
    Cell-Cell Junction Organization
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