Biotin Antikörper

Produktnummer ABIN1043715

Hersteller Produkt- Nr.: 200-301-098

Produktdetails anti-Biotin Antikörper

Target: Biotin

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Biotin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-301-098

Hersteller: Rockland

Verwendungszweck: Biotin Monoclonal Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Produktmerkmale: Synonyms: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R

Aufreinigung: Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).

Immunogen Type: Other

Klon: 4C7-D5

Isotyp: IgG2a lambda

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: 1-2 μg/mL

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000

IF Microscopy Dilution: 2 μg/mL

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Biotin

Target: Biotin

Abstract: Biotin Produkte

Substanzklasse: Chemical

Hintergrund: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.