beta Actin Antikörper (N-Term)

Produktnummer ABIN1043747

Hersteller Produkt- Nr.: 200-301-e69

Produktdetails anti-beta Actin Antikörper

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-301-e69

Hersteller: Rockland

Verwendungszweck: Beta Actin Antibody

Kreuzreaktivität (Details): beta Actin antibody is specific for human and mouse ß Actin protein.

Produktmerkmale: Synonyms: mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Aufreinigung: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.

Immunogen Type: Conjugated Peptide

Klon: 3H2-E4-E12

Isotyp: IgG2a lambda

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:5000-1:50000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Anti-Beta Actin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-beta Actin Antikörper (ABIN1043747)

Carvajal, Vallejos, Walters, Contreras, Hertz, Olivares, Cáceres, Pino, Letelier, Thompson, López-Lastra: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." in: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: ACTB (ACTB Produkte)

Hintergrund: Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Gen-ID: 11461

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction