beta Actin Antikörper (N-Term)

Produktnummer ABIN1043748

Produktdetails anti-beta Actin Antikörper

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Kaninchen, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.

Produktmerkmale: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Sterilität: Sterile filtered

Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Immunogen Type: Peptide

Klon: 3H2-E4-E12

Isotyp: IgG2a lambda

Anwendungsinformationen

Applikationshinweise: Anti-beta Actin antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Kommentare:

Gene Name: Actb

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-beta Actin Antikörper (ABIN1043748)

Carvajal, Vallejos, Walters, Contreras, Hertz, Olivares, Cáceres, Pino, Letelier, Thompson, López-Lastra: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." in: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: beta Actin (ACTB Produkte)

Hintergrund: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Synonyms: Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Gen-ID: 11461

NCBI Accession: NP_031419

UniProt: P60710

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction