JAG1 Antikörper (Internal Region)

Produktnummer ABIN129524

Produktdetails anti-JAG1 Antikörper

Target: JAG1 (Jagged 1 (JAG1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Spezifität: This protein A purified antibody is directed against human Jagged-1 protein. The product was purified from mono-specific antiserum by affinity chromatography. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.

Produktmerkmale: Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.

Aufreinigung: purified

Sterilität: Sterile filtered

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kDa band in human brain and kidney lysates.

Kommentare:

Gene Name: JAG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (WB)

Validierung #103702 (Western Blotting)

by: AG Pancreatic Development and Stem cell differentiation, Universitätsklinikum Ulm

No.: #103702

Datum: 16.06.2019

Antigen: JAG1

Chargennummer: 28285

Validierte Anwendung: Western Blotting

Positivkontrolle:

human pluripotent stem cells (day 0)

pancreatic progenitor cells (day 14)

Negativkontrolle:

definite endoderm cells (day 4)

pancreatic endoderm cells (day 10)

Bewertung:

ABIN1043767 reveals a protein band of the expected MW and some weaker extraneous bands in cell lysates of human pluripotent stem cells and pancreatic progenitor cells.

Validierungsbilder

Western blot with ABIN1043686 on human pluripotent stem cells (1 and 2), definite endoderm cells (3 and 4), pancreatic endoderm cells (5 and 6), and pancreatic progenitors (7 and 8). ABIN1043686 was used at a 1:1000 (30sec exposure) or 1:10000 dilution (5min exposure). Beta-actin served as loading control.

Protokoll

Primärantikörper: ABIN1043767

Sekundärantikörper: donkey anti-rabbit HRP-conjugated antibody (GE Healthcare, NA9310V)

Full Protocol:

• Grow HUES8 in mTeSR1 (STEMCELL Technologies, 85850) at 37°C and 5% O₂, 5% CO₂ in 2ml in a 6-well plate to 90% confluency.
• Harvest cells using TrypLE (ThermoFisher Scientific, 12604013) following the manufacturer´s instructions.
• Lyse 2x10⁶ cells in 30µl per well cold RIPA buffer (50mM Tris-HCl pH 8.0, 150mM NaCl, 0.1% SDS, 0.5% deoxycholate, 1% TritonX 100 in ddH₂O) supplemented with 1mM PMSF and 1x protease inhibitor (Roche, 11836170001) for 30min on ice with 3x vortexing in 1.5ml microcentrifuge tubes.
• Centrifuge tubes at 10000xg for 8min at 4°C.
• Transfer supernatant to a new 1.5ml microcentrifuge tube and store at -80°C.
• Determine total protein content of the lysates using a Bradford assay (Bio-Rad, 500-0006).
• Denature 50µg of total protein for 5min at 95°C in 30µl 1x Laemmli SDS sample buffer and subsequently separate them on a denaturing 7.5% polyacrylamide gel (7.5% Acrylamide, 0.375M Trís-HCl (pH8.8), 0.1% SDS, 0.1% APS, 0.1% TEMED) for about 90min at 120V.
• Transfer proteins onto a PVDF membrane (Sigma Aldrich, IPVH00010) with transfer buffer (5.27g Tris, 2.93 g glycerine, 200 ml methanol, fill to 1l ddH₂O) in a semidry western blotting system for 75min at 80mA/gel.
• Check transfer of the separated proteins by Ponceau S staining.
• Rinse membrane with water.
• Wash membrane for 5min with TBST (TBS, 0.02% Tween20).
• Block the membrane with 20ml blocking buffer (TBST, 5% milk) on a shaker for 1h at RT.
• Rinse membrane 3x with TBST.
• Wash membrane on a shaker 3x for 5min with TBST.
• Shrink-wrap and incubate membrane with primary
• • rabbit anti-JAG1 antibody (antibodies-online, ABIN1043767, lot 28285) diluted 1:1000 and 1:10000 respectively in blocking buffer ON at 4°C or
  • mouse anti-beta actin antibody (Sigma Aldrich, A1978) diluted 1:2000 in blocking buffer at RT for 1h.
• Wash membrane 3x for 5min with TBST.
• Incubate membrane with secondary
• • donkey anti-rabbit HRP-conjugated antibody (GE Healthcare, NA9310V) diluted 1:5000 in TBST containing 1% milk for 1h at RT or
  • donkey anti-mouse HRP-conjugated antibody (GE Healthcare, NA9340V) diluted 1:5000 in TBST containing 1% milk for 1h at RT.
• Wash membrane 3x for 5min with TBST.
• Reveal protein bands using ECL solution (ThermoScientific, 34076) on a Fusion SL (Vilber) chemiluminescence system. Exposure times for the 1:1000 and 1:10000 dilutions of ABIN1043767 were 30sec and 5min respectively.

Anmerkungen:

• The bands marked by the arrow in the western blot image using ABIN1043767 at a 1:1000 dilution seem to be specific, as JAG1 showed highest transcriptomic levels at pancreatic progenitor stage (lanes 7 and 8), followed by pluripotent stem cell state (lane 2). Although the immunoblot with ABIN1043767 results in some unspecific bands, the expected band height fits nicely (75kDa). Higher MW bands might potentially represent precursor proteins detectable in all samples but pancreatic progenitor stage samples. In principle specific bands are also detectable with ABIN1043767 at a 1:10000 dilution, although exposition time was very long.

• ABIN1043767 was also tested in IHC on 4-5µm FFPE sections of primary human kidney cancer tissue. Epitope retrieval was carried out using Tris-EDTA buffer at pH9.0 (Zytomed, ZUC029-500), EDTA at pH8.0 (Leica, RE7116), or citrate buffer at pH6.1 (Agilent, S169984-2) for 20min in a decloaking chamber. Antigen retrieval at pH8.0 looks ok, assuming a broad expression pattern. However, detection would be rather expected in fewer tubular cells. No staining was observed in glomeruli. Cytoplasmic staining in tubuli is revealed at higher magnification. Note that antigen retrieval influences the staining pattern. All in all, staining needs to be validated for example in protein ablation assays.

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-JAG1 Antikörper (ABIN129524)

Boareto, Jolly, Goldman, Pietilä, Mani, Sengupta, Ben-Jacob, Levine, Onuchic: "Notch-Jagged signalling can give rise to clusters of cells exhibiting a hybrid epithelial/mesenchymal phenotype." in: Journal of the Royal Society, Interface, Vol. 13, Issue 118, (2017) (PubMed).

Yabe, Matsumoto, Tsurumoto, Shindo: "Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis." in: Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, Vol. 10, Issue 6, pp. 589-94, (2005) (PubMed).

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: Jagged 1 (JAG1 Produkte)

Synonyme: AGS antikoerper, AHD antikoerper, AWS antikoerper, CD339 antikoerper, HJ1 antikoerper, JAGL1 antikoerper, ABE2 antikoerper, Gsfabe2 antikoerper, Htu antikoerper, Ozz antikoerper, Ser-1 antikoerper, JAG1 antikoerper, jag3 antikoerper, cb243 antikoerper, jag1 antikoerper, sb:cb243 antikoerper, serrateC antikoerper, jagged1 antikoerper, serrate antikoerper, serrate-1 antikoerper, x-serrate-1 antikoerper, jagged 1 antikoerper, jagged 1b antikoerper, jagged 1a antikoerper, jagged 1 L homeolog antikoerper, JAG1 antikoerper, Jag1 antikoerper, jag1 antikoerper, jag1b antikoerper, jag1a antikoerper, jag1.L antikoerper

Hintergrund: Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.
Synonyms: Ser 1 antibody, AGS antibody, AHD antibody, AWS antibody, CD 339 antibody, CD339 antibody, CD339 antigen antibody, Headturner antibody, HJ1 antibody, Htu antibody

Gen-ID: 182, 4557679

UniProt: P78504

Pathways: Notch Signalweg, Stem Cell Maintenance