P35 Antikörper

Produktnummer ABIN1043768

Produktdetails

Target: P35

Reaktivität: Borrelia burgdorferi

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Lateral Flow (LF)

Spezifität: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.

Produktmerkmale: The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34°C but not 24°C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH, being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35-kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease.

Aufreinigung: purified

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.
Immunogen Type: RecombinantProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: BB-H32, bba64

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen

Gautam, Hathaway, McClain, Ramesh, Ramamoorthy: "Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter." in: Microbiology (Reading, England), Vol. 154, Issue Pt 1, pp. 275-85, (2008) (PubMed).

Antigendetails

Target: P35

Synonyme: Anx1 antikoerper, p35 antikoerper, CDK5P35 antikoerper, CDK5R antikoerper, NCK5A antikoerper, p23 antikoerper, p25 antikoerper, p35nck5a antikoerper, 2010323F13Rik antikoerper, GFRP antikoerper, P35 antikoerper, annexin A1 antikoerper, cyclin dependent kinase 5 regulatory subunit 1 antikoerper, GTP cyclohydrolase I feedback regulator antikoerper, Anxa1 antikoerper, CDK5R1 antikoerper, Gchfr antikoerper

Substanzklasse: Viral Protein

Hintergrund: The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34°C but not 24°C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH, being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35-kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease.
Synonyms: bba64, Borrelia burgdorferi p35

Gen-ID: 1194146

NCBI Accession: NP_045522

UniProt: O50687