CCL4 Antikörper (HRP)

Produktnummer ABIN1043803

Hersteller Produkt- Nr.: 209-403-b97

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CCL4 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für CCL4 Antikörper (HRP) (ABIN1043803)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL4 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CCL4 Antikörper (HRP)

Hersteller Produkt- Nr.: 209-403-b97

Hersteller: Rockland

Verwendungszweck: MIP-1 beta Antibody Peroxidase Conjugated

Produktmerkmale: Synonyms: rabbit anti-MIP-1 beta Antibody Peroxidase Conjugated, rabbit anti-MIP-1b Antibody HRP Conjugated, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß

Aufreinigung: Human MIP-1 beta Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogen:

Immunogen: MIP-1 beta Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein-A purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to human MIP-1ß protein by western blotting in the appropriate cell lysate or extract. Immunology Research

Western Blot Dilution: 1:500

ELISA Dilution: 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store MIP-1 beta Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CCL4 Antikörper (HRP) (ABIN1043803)

Guan, Wang, Roderiquez, Norcross: "Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 32348-52, (2002) (PubMed).

Details zu CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Andere Bezeichnung: CCL4

Hintergrund: Background: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. MIP-1 beta Antibody Peroxidase Conjugated is useful for researchers interested in Immunology Research.

Gen-ID: 6351

NCBI Accession: NP_002975

UniProt: P13236