CCL20 Antikörper (HRP)

Produktnummer ABIN1043828

Hersteller Produkt- Nr.: 210-403-b51

Produktdetails anti-CCL20 Antikörper (HRP)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL20 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Hersteller Produkt- Nr.: 210-403-b51

Hersteller: Rockland

Verwendungszweck: MIP-3 alpha Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3α in cell supernatants and certain body fluids.

Produktmerkmale: Synonyms: rabbit anti-MIP-3α peroxidase conjugated antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Aufreinigung: Mouse MIP-3 alpha Antibody Peroxidase Conjugated was heated to 56°C for 30 minutes.

Immunogen:

Immunogen: MIP-3 alpha Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3alpha protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: This purified antibody has been tested in western blotting and is suitable for ELISA. By western blot a band approximately 10.7 kDa in size corresponding to mouse MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store MIP-3 alpha Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CCL20 Antikörper (HRP) (ABIN1043828)

Harper, Guo, Rizzo, Lillis, Kurtz, Skorcheva, Purdy, Fitch, Iordanov, Blauvelt: "Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis." in: The Journal of investigative dermatology, Vol. 129, Issue 9, pp. 2175-83, (2009) (PubMed).

Details zu CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: Ccl20 (CCL20 Produkte)

Hintergrund: Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Gen-ID: 20297

NCBI Accession: NP_058656

UniProt: O89093

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion