Glycogen Synthase 1 Antikörper (pSer640)

Produktnummer ABIN1043894

Produktdetails anti-Glycogen Synthase 1 Antikörper

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Bindungsspezifität: pSer640

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glycogen Synthase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Spezifität: This affinity purified antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.

Produktmerkmale: Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephos-phorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phos-phorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.

Sterilität: Sterile filtered

Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This phospho specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Kommentare:

Gene Name: GYS1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-Glycogen Synthase 1 Antikörper (ABIN1043894)

Kelsall, Voss, Munro, Cuthbertson, Cohen: "R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals." in: Journal of neurochemistry, Vol. 118, Issue 4, pp. 596-610, (2011) (PubMed).

Details zu Glycogen Synthase 1

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Andere Bezeichnung: Muscle Glycogen Synthase (GYS1 Produkte)

Synonyme: GSY antikoerper, GYS antikoerper, GYS1 antikoerper, zgc:63701 antikoerper, GYS2 antikoerper, Gys3 antikoerper, MGS antikoerper, glycogen synthase 1 antikoerper, glycogen synthase 1 (muscle) antikoerper, glycogen synthase 1, muscle antikoerper, GYS1 antikoerper, gys1 antikoerper, Gys1 antikoerper

Hintergrund: Anti-Glycogen Synthase 1 pS640 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephos-phorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phos-phorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.
Synonyms: Glycogen antibody, Glycogen synthase 1 (muscle) antibody, Glycogen synthase 1 antibody, Glycogen synthase1 antibody, GSY antibody, GYS 1 antibody, GYS antibody, GYS1 antibody, Starchsynthase muscle antibody

Gen-ID: 2997

UniProt: P13807

Pathways: PI3K-Akt Signalweg, AMPK Signaling, Cellular Glucan Metabolic Process