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NYS48/HAUS8 Antikörper (Ser70)

Rockland 600-401-c53 HAUS8 Reaktivität: Human WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN1043902
Hersteller Produkt- Nr.: 600-401-c53
  • Target Alle NYS48/HAUS8 (HAUS8) Antikörper anzeigen
    NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
    Bindungsspezifität
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser70
    Reaktivität
    • 24
    • 5
    • 2
    • 1
    Human
    Wirt
    • 23
    • 2
    Kaninchen
    Klonalität
    • 25
    Polyklonal
    Konjugat
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NYS48/HAUS8 Antikörper ist unkonjugiert
    Applikation
    • 23
    • 13
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA
    Hersteller Produkt- Nr.
    600-401-c53
    Hersteller
    Rockland
    Verwendungszweck
    HICE1 Antibody
    Kreuzreaktivität (Details)
    Anti-Hice1 Affinity purified antibody is directed against human Hice1 protein.
    Produktmerkmale
    Synonyms: rabbit anti-HICE1 antibody, HICE-1, HICE 1, HAUS8, HAUS-8, HAUS 8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
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  • Applikationshinweise

    Application Note: Hice1 antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

    Western Blot Dilution: 0.45 μg/mL

    ELISA Dilution: 1:30,000-1:45,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.45 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Wu, Lin, Wei, Chen, Shan, Lee: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).

  • Target
    NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
    Andere Bezeichnung
    HAUS8 (HAUS8 Produkte)
    Hintergrund
    Background: Hice1 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
    Gen-ID
    93323
    NCBI Accession
    NP_219485
    UniProt
    Q9BT25
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