SPRY2 Antikörper (Internal Region)

Produktnummer ABIN1043909

Produktdetails anti-SPRY2 Antikörper

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPRY2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Verwendungszweck: Sprouty-2 Antibody

Kreuzreaktivität (Details): Sprouty-2 antibody reacts with human Sprouty 2 protein.

Produktmerkmale: Synonyms: Rabbit anti-Sprouty2 antibody, Rabbit anti-Sprouty-2 antibody, Sprouty 2, SPRY2, SPRY 2, Spry-2, Protein sprouty homolog 2

Aufreinigung: Anti-Sprouty-2 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Sprouty-2 affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 10 μg/mL

Application Note: Anti-Sprouty-2 antibody has been tested by ELISA, Immunofluorescence, Immunohistochemistry, and is suitable for western blotting. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:5,000

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.04 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-SPRY2 Antikörper (ABIN1043909)

Zhang, Chaturvedi, Jaggar, Magnuson, Lee, Patel: "Regulation of vascular smooth muscle cell proliferation and migration by human sprouty 2." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 3, pp. 533-8, (2005) (PubMed).

August: "Cerebrovascular and carotid artery disease." in: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics, Vol. 92, Issue 3, pp. 253-6, (2001) (PubMed).

Details zu SPRY2

Target: SPRY2 (Sprouty Homolog 2 (SPRY2))

Andere Bezeichnung: SPRY2 (SPRY2 Produkte)

Hintergrund: Background: Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein. Significant sequence divergence occurs at the N-terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Gen-ID: 10253

UniProt: O43597

Pathways: EGFR Signaling Pathway, Sensory Perception of Sound, EGFR Downregulation