GST Antikörper (FITC)

Produktnummer ABIN1043914

Hersteller Produkt- Nr.: 600-402-200

Produktdetails anti-GST Antikörper (FITC)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-402-200

Hersteller: Rockland

Verwendungszweck: GST Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Produktmerkmale: Synonyms: rabbit anti-GST antibody fluorescein conjugation, FITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Aufreinigung: FITC conjugated GST Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 3.0

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Application Note: FITC conjugated GST Antibody has been tested by western blot and is ideal for microscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:60,000

IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store FITC conjugated GST Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST (GST Produkte)

Hintergrund: Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.