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GDF15 Antikörper (C-Term) (Biotin)

Rockland 600-406-b07 GDF15 Reaktivität: Human, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal Biotin
Rockland
Produktnummer ABIN1043920
Hersteller Produkt- Nr.: 600-406-b07
  • Target Alle GDF15 Antikörper anzeigen
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Bindungsspezifität
    • 15
    • 11
    • 10
    • 7
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 88
    • 27
    • 21
    • 1
    • 1
    Human, Maus
    Wirt
    • 81
    • 14
    • 3
    • 1
    Kaninchen
    Klonalität
    • 81
    • 17
    • 1
    Polyklonal
    Konjugat
    • 52
    • 11
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GDF15 Antikörper ist konjugiert mit Biotin
    Applikation
    • 78
    • 46
    • 34
    • 13
    • 13
    • 13
    • 7
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    600-406-b07
    Hersteller
    Rockland
    Verwendungszweck
    GDF15 Antibody Biotin Conjugated
    Kreuzreaktivität (Details)
    This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues.
    Produktmerkmale
    Synonyms: rabbit anti-NAG1 antibody biotin conjugation, biotin conjugated rabbit anti-NAG1 antibody, NAG 1, NAG-1, GDF15, MIC1, MIC-1, GDF-15, PLAB, PTGFB, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, Placental bone morphogenetic protein, Prostate differentiation factor
    Aufreinigung
    Nag-1 Antibody Biotin Conjugated antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Immunogen

    Immunogen: Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
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  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: NAG1/GDF15 Antibody Biotin Conjugated antibody has been tested by ELISA and western blotting of human and mouse NAG-1 protein.  For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

    Western Blot Dilution: 1:2,000-1:10,000

    ELISA Dilution: 1:20,000-1:100,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).

  • Target
    GDF15 (Growth Differentiation Factor 15 (GDF15))
    Andere Bezeichnung
    GDF15 (GDF15 Produkte)
    Hintergrund
    Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
    Gen-ID
    9518
    UniProt
    Q99988
    Pathways
    SARS-CoV-2 Protein Interaktom
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