Myosin Antikörper (pSer19, pSer20)

Produktnummer ABIN104491

Produktdetails anti-Myosin Antikörper

Target: Myosin

Bindungsspezifität: pSer19, pSer20

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myosin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This phospho specific polyclonal antibody was tested by ELISA, immunohistochemistry and immunoblotting.   Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes.  The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional by immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.92 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Myosin Antikörper (ABIN104491)

Harbom, Rudisill, Michel, Litwa, Beenhakker, McConnell: "The effect of rho kinase inhibition on morphological and electrophysiological maturity in iPSC-derived neurons." in: Cell and tissue research, Vol. 375, Issue 3, pp. 641-654, (2019) (PubMed).

Salomon, Gaston, Magescas, Duvauchelle, Canioni, Sengmanivong, Mayeux, Michaux, Campeotto, Lemale, Viala, Poirier, Minc, Schmitz, Brousse, Ladoux, Goulet, Delacour: "Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity." in: Nature communications, Vol. 8, pp. 13998, (2018) (PubMed).

Panousopoulou, Hobbs, Mason, Green, Formstone: "Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body." in: Journal of cell science, Vol. 129, Issue 9, pp. 1915-27, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." in: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Newell-Litwa, Badoual, Asmussen, Patel, Whitmore, Horwitz: "ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity." in: The Journal of cell biology, Vol. 210, Issue 2, pp. 225-42, (2016) (PubMed).

Roland, Ricobaraza, Carrel, Jordan, Rico, Simon, Humbert-Claude, Ferrier, McFadden, Scheuring, Lenkei: "Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth." in: eLife, Vol. 3, pp. e03159, (2015) (PubMed).

Solanes, Heuzé, Maurin, Bretou, Lautenschlaeger, Maiuri, Terriac, Thoulouze, Launay, Piel, Vargas, Lennon-Duménil: "Space exploration by dendritic cells requires maintenance of myosin II activity by IP3 receptor 1." in: The EMBO journal, Vol. 34, Issue 6, pp. 798-810, (2015) (PubMed).

Fabbiano, Menacho-Márquez, Sevilla, Albarrán-Juárez, Zheng, Offermanns, Montero, Bustelo: "Genetic dissection of the vav2-rac1 signaling axis in vascular smooth muscle cells." in: Molecular and cellular biology, Vol. 34, Issue 24, pp. 4404-19, (2015) (PubMed).

Edelson, Brautigan: "The Discodermia calyx toxin calyculin a enhances cyclin D1 phosphorylation and degradation, and arrests cell cycle progression in human breast cancer cells." in: Toxins, Vol. 3, Issue 1, pp. 105-19, (2013) (PubMed).

Details zu Myosin

Target: Myosin

Abstract: Myosin Produkte

Synonyme: CG3201 antikoerper, DmMLC_C antikoerper, Dmel\\CG3201 antikoerper, MyoII antikoerper, mlc-c antikoerper, nmELC antikoerper, MYOSIN antikoerper, myosin 1 antikoerper, DFNA4 antikoerper, DFNA4A antikoerper, MHC16 antikoerper, MYH17 antikoerper, NMHC II-C antikoerper, NMHC-II-C antikoerper, PNMHH antikoerper, myosin antikoerper, MYH1 antikoerper, MYH2 antikoerper, MYH3 antikoerper, MYH4 antikoerper, MYH8 antikoerper, adult antikoerper, embryonic antikoerper, 2400004E04Rik antikoerper, II-C antikoerper, NHMCII antikoerper, DROMHC antikoerper, Dm nmII antikoerper, DmnmII antikoerper, Dronm-MII antikoerper, E(br) antikoerper, MHC antikoerper, Mhc-c antikoerper, Mhc-c[1] antikoerper, Myo antikoerper, Myo II antikoerper, NMM antikoerper, Zip antikoerper, anon-WO0140519.37 antikoerper, l(2)02957 antikoerper, nmMHC antikoerper, nmy-2 antikoerper, zpr antikoerper, DmelCG15792 antikoerper, CG15792 antikoerper, Myosin light chain cytoplasmic antikoerper, hypothetical protein antikoerper, myosin 1 antikoerper, myosin heavy chain 9 antikoerper, myosin heavy chain 14 antikoerper, myosin, heavy chain 1B, skeletal muscle antikoerper, myosin, heavy polypeptide 14 antikoerper, zipper antikoerper, Mlc-c antikoerper, T22C1.6 antikoerper, ATM1 antikoerper, MYH9 antikoerper, MYH14 antikoerper, Myh14 antikoerper, MYH1B antikoerper, zip antikoerper

Hintergrund: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  This antibody is specific for the phosphorylated form of myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site.  Human and mouse have almost identical sequences.  In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
Synonyms: Myosin regulatory light chain 12A Alternative name(s): Myosin regulatory light chain MRLC3 Myosin regulatory light chain 2, nonsarcomeric Myosin RLC MLC-2B

Gen-ID: 10627

UniProt: P19105