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5HT1B Receptor Antikörper (Internal Region)

HTR1B Reaktivität: Human, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048254
  • Target Alle 5HT1B Receptor (HTR1B) Antikörper anzeigen
    5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))
    Bindungsspezifität
    • 9
    • 7
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 38
    • 16
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Affe
    Wirt
    • 35
    • 2
    • 1
    Kaninchen
    Klonalität
    • 37
    • 1
    Polyklonal
    Konjugat
    • 19
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser 5HT1B Receptor Antikörper ist unkonjugiert
    Applikation
    • 28
    • 25
    • 8
    • 7
    • 3
    • 3
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human 5HT1B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Bat, Horse, Pig (94%) Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig (88%) Elephant, Opossum (82%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from internal region of human 5HT1B Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Bat, Horse, Pig (94%), Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig (88%), Elephant, Opossum (82%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))
    Andere Bezeichnung
    HTR1B / 5-HT1B Receptor (HTR1B Produkte)
    Synonyme
    5-HT1B antikoerper, 5-HT1DB antikoerper, HTR1D2 antikoerper, HTR1DB antikoerper, S12 antikoerper, 5-HT1Db antikoerper, 5-HT1-LIKE antikoerper, HTR1B antikoerper, 5-HT-dro2B antikoerper, 5-HT1BDro antikoerper, 5-HT[1B]Dro antikoerper, 5-HT[[1BDro]] antikoerper, 5-HT[[1B]] antikoerper, 5-HT[[1B]]DRO antikoerper, 5-HT[[1B]]Dro antikoerper, 5-HT[[dro2B]] antikoerper, 5HT-R2B antikoerper, 5HT-dro2B antikoerper, 5HT-dro[[2B]] antikoerper, 5HT1B antikoerper, 5htdro2b antikoerper, CG15113 antikoerper, CG30126 antikoerper, DRO2B antikoerper, Dm5HTdro2B antikoerper, Dmel\\CG15113 antikoerper, Dmel_CG30126 antikoerper, d5-HT1B antikoerper, 5-HT-1B antikoerper, zgc:194666 antikoerper, zgc:194690 antikoerper, 5-hydroxytryptamine receptor 1B antikoerper, 5-hydroxytryptamine (serotonin) receptor 1B antikoerper, HTR1B antikoerper, Htr1b antikoerper, 5-HT1B antikoerper, htr1b antikoerper
    Hintergrund
    Name/Gene ID: HTR1B
    Subfamily: Serotonin
    Family: GPCR

    Synonyms: HTR1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1b receptor, 5-HT1DB, 5HT1B Receptor, HTR1DB, Serotonin 5-HT-1b receptor, Serotonin receptor 1B, Serotonin 1b receptor, HTR1D2, Serotonin 1D beta receptor
    Gen-ID
    3351
    Pathways
    JAK-STAT Signalweg, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteine
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