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5HT1D Antikörper (Cytoplasmic Domain)

HTR1D Reaktivität: Human, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048256
  • Target Alle 5HT1D (HTR1D) Antikörper anzeigen
    5HT1D (HTR1D) (Serotonin Receptor 1D (HTR1D))
    Bindungsspezifität
    • 15
    • 8
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 38
    • 18
    • 10
    • 1
    Human, Affe
    Wirt
    • 45
    Kaninchen
    Klonalität
    • 45
    Polyklonal
    Konjugat
    • 14
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser 5HT1D Antikörper ist unkonjugiert
    Applikation
    • 19
    • 18
    • 13
    • 13
    • 9
    • 3
    • 3
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human 5HT1D Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Panda, Bovine, Bat, Horse, Rabbit, Pig, Guinea pig, Turkey, Chicken (94%) Hamster, Elephant, Opossum (88%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1D Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Panda, Bovine, Bat, Horse, Rabbit, Pig, Guinea pig, Turkey, Chicken (94%), Hamster, Elephant, Opossum (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product HTR1D Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (17 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    5HT1D (HTR1D) (Serotonin Receptor 1D (HTR1D))
    Andere Bezeichnung
    HTR1D / 5-HT1D Receptor (HTR1D Produkte)
    Synonyme
    AI853647 antikoerper, Gpcr14 antikoerper, Htr1db antikoerper, 5-HT1D antikoerper, HT1DA antikoerper, HTR1DA antikoerper, HTRL antikoerper, RDC4 antikoerper, 5HT1D antikoerper, 5-HT-1D antikoerper, 5-hydroxytryptamine (serotonin) receptor 1D antikoerper, 5-hydroxytryptamine receptor 1D antikoerper, Htr1d antikoerper, HTR1D antikoerper
    Hintergrund
    Name/Gene ID: HTR1D
    Subfamily: Serotonin
    Family: GPCR

    Synonyms: HTR1D, 5-HT1d alpha receptor, 5HT1D Receptor, 5-HT1d-type serotonin receptor, 5-HT1D, 5-HT-1D, 5-HT-1D-alpha, 5-HT1d receptor, HT1d receptor, HTR1DA, HTRL, HT1DA, Serotonin receptor 1D, RDC4, Gpcr14, Serotonin 1d receptor, Serotonin 5-HT-1d receptor
    Gen-ID
    3352
    Pathways
    JAK-STAT Signalweg
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