HTR2A Antikörper (C-Term)
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- Target Alle HTR2A Antikörper anzeigen
- HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser HTR2A Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human 5HT2A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Orangutan, Gibbon, Monkey (95%) Marmoset (85%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 20 amino acid peptide from C-terminal cytoplasmic domainhuman 5HT2A Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Gibbon, Monkey (95%), Marmoset (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HTR2A Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Antigen retrieval requires Proteinase K treatment. - Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
- Andere Bezeichnung
- HTR2A / 5-HT2A Receptor (HTR2A Produkte)
- Synonyme
- 5-HT2A antikoerper, HTR2 antikoerper, 5-HT-2 antikoerper, 5-HT-2A antikoerper, 5-HTR2A antikoerper, LOC100009461 antikoerper, E030013E04 antikoerper, Htr-2 antikoerper, Htr2 antikoerper, 5Ht-2 antikoerper, 5HT2A antikoerper, 5HTR2A antikoerper, HTR2A antikoerper, Htr2a antikoerper, 5HT2 antikoerper, 5-hydroxytryptamine receptor 2A antikoerper, 5-hydroxytryptamine (serotonin) receptor 2A antikoerper, HTR2A antikoerper, Htr2a antikoerper
- Hintergrund
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Name/Gene ID: HTR2A
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR2A, 5-HT2A, 5-HT-2A, 5-HT2a receptor, 5HT2A Receptor, 5-HT-2, 5-HT2A/2C, 5-HT2A/2C receptor, 5-HT2 receptor, HTR2, Serotonin 5-HT-2A receptor, Serotonin receptor 2A, Serotonin 2a receptor - Gen-ID
- 3356
- Pathways
- JAK-STAT Signalweg, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process
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