Serotonin Receptor 4 Antikörper (C-Term)

Produktnummer ABIN1048275

Produktdetails anti-Serotonin Receptor 4 Antikörper

Target: Serotonin Receptor 4 (HTR4)

Bindungsspezifität: C-Term

Reaktivität: Human, Schwein, Pferd, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human 5HT4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Horse, Pig (100%) Panda, Dog, Rabbit (95%) Bat, Bovine (90%) Guinea pig (85%) Mouse, Rat (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from C-terminus of human 5HT4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Horse, Pig (100%), Panda, Dog, Rabbit (95%), Bat, Bovine (90%), Guinea pig (85%), Mouse, Rat (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (7 - 17 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Andere Bezeichnung: HTR4 / 5-HT4 Receptor (HTR4 Produkte)

Synonyme: 5-HT4 antikoerper, 5-HT<4L> antikoerper, 5HTR4 antikoerper, 5-HT4S antikoerper, 5-HT4R antikoerper, 5 hydroxytryptamine (serotonin) receptor 4 antikoerper, 5-hydroxytryptamine receptor 4 antikoerper, Htr4 antikoerper, HTR4 antikoerper

Hintergrund: Name/Gene ID: HTR4
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR4, 5-HT4, 5-HT4R, 5-HT-4, 5HT4 receptor, 5-HT4 receptor, 5-hydroxytryptamine 4 receptor, 5-hydroxytryptamine receptor 4, Serotonin 5-HT-4a receptor, Serotonin receptor 4, Cardiac 5-HT4 receptor, Serotonin 4 receptor, Serotonin 5-HT-4 receptor

Gen-ID: 3360

Pathways: JAK-STAT Signalweg, cAMP Metabolic Process, S100 Proteine