ADORA1 Antikörper (C-Term)

Produktnummer ABIN1048301

Produktdetails anti-ADORA1 Antikörper

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADORA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human Adenosine A1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%) Orangutan, Marmoset, Elephant, Horse (95%) Rat, Pig, Guinea pig (90%) Bovine, Hamster, Panda (85%) Mouse, Dog (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from C-terminus of human Adenosine A1 Receptor. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%), Orangutan, Marmoset, Elephant, Horse (95%), Rat, Pig, Guinea pig (90%), Bovine, Hamster, Panda (85%), Mouse, Dog (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (7.2 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Andere Bezeichnung: ADORA1 / Adenosine A1 Receptor (ADORA1 Produkte)

Synonyme: adora1-A antikoerper, a1R antikoerper, ADORA1 antikoerper, LOC606743 antikoerper, LOC100222054 antikoerper, RDC7 antikoerper, A1-AR antikoerper, A1AR antikoerper, A1R antikoerper, AA1R antikoerper, AI848715 antikoerper, BB176431 antikoerper, Ri antikoerper, adenosine A1 receptor L homeolog antikoerper, adenosine A1 receptor antikoerper, adenosine receptor A1 antikoerper, adora1.L antikoerper, ADORA1 antikoerper, adora1 antikoerper, LOC100222054 antikoerper, Adora1 antikoerper

Hintergrund: Name/Gene ID: ADORA1
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA1, A1 adenosine receptor, A1ar, Adenosine A1 receptor, Adenosine receptor A1, ADO-A1 receptor, RDC7

Gen-ID: 134

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane