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ADRB3 Antikörper (N-Term)

ADRB3 Reaktivität: Human, Ratte IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048320
  • Target Alle ADRB3 Antikörper anzeigen
    ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))
    Bindungsspezifität
    • 15
    • 15
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 57
    • 36
    • 25
    Human, Ratte
    Wirt
    • 55
    • 2
    • 2
    • 2
    Kaninchen
    Klonalität
    • 61
    Polyklonal
    Konjugat
    • 23
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ADRB3 Antikörper ist unkonjugiert
    Applikation
    • 46
    • 26
    • 26
    • 18
    • 9
    • 6
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human ADRB3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon (95%) Guinea pig (90%) Monkey, Elephant, Rabbit (85%) Sheep, Goat, Hamster, Cat, Bovine, Horse (80%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 20 amino acid peptide from N-terminal extracellular domain of human ADRB3. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon (95%), Guinea pig (90%), Monkey, Elephant, Rabbit (85%), Sheep, Goat, Hamster, Cat, Bovine, Horse (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product ADRB3 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (17 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))
    Andere Bezeichnung
    ADRB3 (ADRB3 Produkte)
    Synonyme
    BETA3AR antikoerper, Adrb-3 antikoerper, ADRB antikoerper, BAR3 antikoerper, B3AR antikoerper, GPCR antikoerper, im:7154935 antikoerper, si:ch211-215f19.1 antikoerper, zgc:194843 antikoerper, zgc:194852 antikoerper, adrenoceptor beta 3 antikoerper, adrenergic receptor, beta 3 antikoerper, adrenoceptor beta 3a antikoerper, adrenoceptor beta 3b antikoerper, ADRB3 antikoerper, Adrb3 antikoerper, adrb3a antikoerper, adrb3b antikoerper
    Hintergrund
    Name/Gene ID: ADRB3
    Subfamily: Adrenergic
    Family: GPCR

    Synonyms: ADRB3, Adrenoceptor beta 3, ADRB3R, Adrenaline beta 3 receptor, Adrenergic, beta-3-, receptor, Beta-3 adrenergic receptor, B3AR, Beta-3 adrenoceptor, Beta3-AR, Beta-3 adrenoreceptor, BETA3AR
    Gen-ID
    155
    Pathways
    cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation
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