Apelin Receptor Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048325

Produktdetails anti-Apelin Receptor Antikörper

Target: Apelin Receptor (APLNR)

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Pferd, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Apelin Receptor Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human Apelin Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Horse (100%) Orangutan, Marmoset, Mouse, Rat, Bat, Rabbit (94%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human Apelin Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Horse (100%), Orangutan, Marmoset, Mouse, Rat, Bat, Rabbit (94%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 - 7 μg/mL)

Not recommended for: WB

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Apelin Receptor

Target: Apelin Receptor (APLNR)

Andere Bezeichnung: APLNR/ Apelin Receptor / APJ (APLNR Produkte)

Synonyme: AGTRL1 antikoerper, APJ antikoerper, APJR antikoerper, HG11 antikoerper, Agtrl1 antikoerper, msr/apj antikoerper, Apj antikoerper, X-msr antikoerper, Xangio1 antikoerper, Xmsr antikoerper, agtrl1 antikoerper, angio1 antikoerper, apj antikoerper, apjr antikoerper, hg11 antikoerper, msr antikoerper, APLNR antikoerper, DKFZp469C1532 antikoerper, agtrl1-B antikoerper, agtrl1b antikoerper, Agtrl1a antikoerper, grn antikoerper, zgc:114063 antikoerper, APJa antikoerper, XAngio1 antikoerper, agtrl1-A antikoerper, agtrl1a antikoerper, aplnr-a antikoerper, aplnr-b antikoerper, x-MSR antikoerper, xAngio1 antikoerper, APLNR1 antikoerper, apelin receptor antikoerper, apelin receptor S homeolog antikoerper, apelin receptor b antikoerper, apelin receptor L homeolog antikoerper, APLNR antikoerper, Aplnr antikoerper, aplnr antikoerper, aplnr.S antikoerper, aplnrb antikoerper, aplnr.L antikoerper

Hintergrund: Name/Gene ID: APLNR
Subfamily: Apelin
Family: GPCR

Synonyms: APLNR, AGTRL1, Angiotensin receptor-like 1, Angiotensin II receptor-like 1, Apelin receptor, APJ, APJ (apelin) receptor, APJR, G protein-coupled receptor APJ, HG11 orphan receptor, HG11, APJ receptor, G-protein coupled receptor APJ

Gen-ID: 187