Bestrophin 2 Antikörper (Internal Region)

Produktnummer ABIN1048351

Produktdetails anti-Bestrophin 2 Antikörper

Target: Bestrophin 2 (BEST2)

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Affe, Schwein, Rind (Kuh), Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Bestrophin 2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human BEST2 / Bestrophin-2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except BEST3 (59 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Bovine, Pig (100%) Panda, Dog, Bat, Rabbit, Opossum (94%) Marmoset (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from internal region of human BEST2 / Bestrophin-2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Bovine, Pig (100%), Panda, Dog, Bat, Rabbit, Opossum (94%), Marmoset (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 - 5 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Bestrophin 2

Target: Bestrophin 2 (BEST2)

Andere Bezeichnung: BEST2 / Bestrophin-2 (BEST2 Produkte)

Synonyme: VMD2L1 antikoerper, CG10173 antikoerper, Dmel\\CG10173 antikoerper, dbest2 antikoerper, Vmd2l1 antikoerper, best2 antikoerper, vmd2l1 antikoerper, MGC53680 antikoerper, MGC132361 antikoerper, Best2 antikoerper, GB16861 antikoerper, si:dkey-121b10.4 antikoerper, MGC75942 antikoerper, best2-a antikoerper, best2-b antikoerper, variant 2 antikoerper, bestrophin 2 antikoerper, Bestrophin 2 antikoerper, bestrophin 2 L homeolog antikoerper, uncharacterized LOC408898 antikoerper, bestrophin 2 S homeolog antikoerper, BEST2 antikoerper, Best2 antikoerper, best2.L antikoerper, LOC408898 antikoerper, best2 antikoerper, best2.S antikoerper

Hintergrund: Name/Gene ID: BEST2
Subfamily: Bestrophin
Family: Ion channel

Synonyms: BEST2, Bestrophin-2, Bestrophin 2, Hbest2, VMD2-like gene 1, VMD2L1

Gen-ID: 54831