CALCRL Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048377

Produktdetails anti-CALCRL Antikörper

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Ratte, Pferd, Kaninchen, Rind (Kuh), Hund, Ziege, Affe, Xenopus laevis, Hamster, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CALCRL Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CALCRL. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CALCR (56 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%) Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%) Turkey, Chicken (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CALCRL. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%), Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%), Turkey, Chicken (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (34 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CALCRL Antikörper (ABIN1048377)

Zheng, Nixdorf-Bergweiler, van Brederode, Alzheimer, Messlinger: "Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices." in: International journal of molecular sciences, Vol. 22, Issue 7, (2021) (PubMed).

Details zu CALCRL

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Andere Bezeichnung: CALCRL / CGRP Receptor (CALCRL Produkte)

Synonyme: CALCRL antikoerper, CGRPR antikoerper, CRLR antikoerper, AV071593 antikoerper, CLR antikoerper, Crlr antikoerper, RATCRLR antikoerper, RNCLR antikoerper, calcitonin receptor like receptor antikoerper, calcitonin receptor-like antikoerper, calcitonin receptor like receptor L homeolog antikoerper, CALCRL antikoerper, LOC100168906 antikoerper, calcrl antikoerper, Calcrl antikoerper, calcrl.L antikoerper

Hintergrund: Name/Gene ID: CALCRL
Subfamily: Adrenomedullin
Family: GPCR

Synonyms: CALCRL, CGRP type 1 receptor, Cgrp1 receptor, CRLR, Calcitonin receptor-like, CGRP receptor, CGRPR

Gen-ID: 10203

Pathways: cAMP Metabolic Process