CCR2 Antikörper (N-Term)

Produktnummer ABIN1048390

Produktdetails anti-CCR2 Antikörper

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Bindungsspezifität: N-Term

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCR2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CCR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human CCR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3 - 30 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CCR2

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Andere Bezeichnung: CCR2 (CCR2 Produkte)

Synonyme: CC-CKR-2 antikoerper, CCR-2 antikoerper, CCR2A antikoerper, CCR2B antikoerper, CD192 antikoerper, CKR2 antikoerper, CKR2A antikoerper, CKR2B antikoerper, CMKBR2 antikoerper, MCP-1-R antikoerper, ACKR5 antikoerper, CKRX antikoerper, CRAM antikoerper, CRAM-A antikoerper, CRAM-B antikoerper, HCR antikoerper, Cc-ckr-2 antikoerper, Ccr2a antikoerper, Ccr2b antikoerper, Ckr2 antikoerper, Ckr2a antikoerper, Ckr2b antikoerper, Cmkbr2 antikoerper, mJe-r antikoerper, C-C motif chemokine receptor 2 antikoerper, C-C motif chemokine receptor like 2 antikoerper, chemokine (C-C motif) receptor 2 antikoerper, CCR2 antikoerper, CCRL2 antikoerper, Ccr2 antikoerper, LOC484790 antikoerper

Hintergrund: Name/Gene ID: CCR2
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR2, C-C chemokine receptor type 2, CD192, Cc chemokine receptor 2, CCR-2, CCR2A, C-C CKR-2, CKR2, CD192 antigen, CKR2A, CKR2B, Mcp1 receptor, MCP-1 receptor, MCP-1-R, C-c chemokine receptor 2, CC-CKR-2, Cc-ckr2, CCR2B, CMKBR2, Mcp1r

Gen-ID: 729230

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process