CCRL1 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048408

Produktdetails anti-CCRL1 Antikörper

Target: CCRL1 (Chemokine (C-C Motif) Receptor-Like 1 (CCRL1))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Maus, Ratte, Kaninchen, Pferd, Hund, Schwein, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCRL1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CCRL1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CCR6 (63 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig (100%) Marmoset, Bovine, Hamster, Opossum (94%) Turkey, Chicken, Xenopus (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human CCRL1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig (100%), Marmoset, Bovine, Hamster, Opossum (94%), Turkey, Chicken, Xenopus (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (24 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CCRL1

Target: CCRL1 (Chemokine (C-C Motif) Receptor-Like 1 (CCRL1))

Andere Bezeichnung: ACKR4 / CCRL1 / CCR11 (CCRL1 Produkte)

Synonyme: CC-CKR-11 antikoerper, CCBP2 antikoerper, CCR-11 antikoerper, CCR10 antikoerper, CCR11 antikoerper, CCRL1 antikoerper, CCX CKR antikoerper, CCX-CKR antikoerper, CKR-11 antikoerper, PPR1 antikoerper, VSHK1 antikoerper, A630091E18Rik antikoerper, CCX-CKR1 antikoerper, Ccrl1 antikoerper, Cmkbrl1 antikoerper, atypical chemokine receptor 4 antikoerper, chemokine (C-C motif) receptor-like 1 antikoerper, Chemokine receptor-like 1 antikoerper, chemerin chemokine-like receptor 1 antikoerper, ACKR4 antikoerper, Ackr4 antikoerper, CCRL1 antikoerper, cml1 antikoerper, CMKLR1 antikoerper

Hintergrund: Name/Gene ID: ACKR4
Subfamily: Chemokine
Family: GPCR

Synonyms: ACKR4, C-C CKR-11, CC-CKR-11, CC chemokine receptor-like 1, CCR11, CCX CKR, C-C chemokine receptor type 11, CKR-11, CCRL1, VSHK1, CCR-11, CCX-CKR, PPR1

Gen-ID: 51554