CDC7 Antikörper (Kinase Domain)
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- Target Alle CDC7 Antikörper anzeigen
- CDC7 (Cell Division Cycle 7 (CDC7))
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Bindungsspezifität
- Kinase Domain
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Reaktivität
- Human, Hund, Pferd, Rind (Kuh), Kaninchen, Fledermaus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CDC7 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human CDC7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%) Monkey, Mouse, Rat, Hamster, Platypus (95%) Turkey, Chicken, Lizard, Xenopus (89%) Stickleback, Pufferfish, Zebrafish (84%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 19 amino acid peptide from kinase domain of human CDC7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%), Monkey, Mouse, Rat, Hamster, Platypus (95%), Turkey, Chicken, Lizard, Xenopus (89%), Stickleback, Pufferfish, Zebrafish (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CDC7 Primärantikörper
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- Applikationshinweise
- Approved: IHC, IHC-P (6.5 μg/mL)
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- CDC7 (Cell Division Cycle 7 (CDC7))
- Andere Bezeichnung
- CDC7 (CDC7 Produkte)
- Synonyme
- CDC7L1 antikoerper, HsCDC7 antikoerper, Hsk1 antikoerper, huCDC7 antikoerper, AI597260 antikoerper, Cdc7l1 antikoerper, muCdc7 antikoerper, cell division cycle 7 antikoerper, cell division cycle 7-related protein kinase antikoerper, cell division cycle 7 (S. cerevisiae) antikoerper, CDC7 antikoerper, Tsp_08585 antikoerper, Cdc7 antikoerper
- Hintergrund
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Name/Gene ID: CDC7
Subfamily: CDC7
Family: Protein Kinase
Synonyms: CDC7, Cdc7 Kinase, CDC7-like 1, CDC7L1, HsCDC7, HuCDC7, CDC7-related kinase, Hsk1, MuCdc7 - Gen-ID
- 8317
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication
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