CDK12 Antikörper (Internal Region)

Produktnummer ABIN1048422

Produktdetails anti-CDK12 Antikörper

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Bindungsspezifität: Internal Region

Reaktivität: Human, Hund, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK12 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CDK12 / CRKRS. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except AKAP12 (55 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (100%) Elephant, Bovine, Rabbit (95%) Rat, Bat (90%) Mouse, Hamster, Horse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from internal region of human CDK12 / CRKRS. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (100%), Elephant, Bovine, Rabbit (95%), Rat, Bat (90%), Mouse, Hamster, Horse (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (6 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CDK12

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Andere Bezeichnung: CDK12 / CRKRS (CRKRS Produkte)

Synonyme: AC017581 antikoerper, CG7597 antikoerper, Dmel\\CG7597 antikoerper, anon-WO0140519.165 antikoerper, cg7597 antikoerper, dCDK12 antikoerper, gi24668141 antikoerper, CRK7 antikoerper, CRKR antikoerper, CRKRS antikoerper, hCDK12 antikoerper, 1810022J16Rik antikoerper, AI646528 antikoerper, Crk7 antikoerper, Crkrs antikoerper, D11Ertd752e antikoerper, Pksc antikoerper, Cyclin-dependent kinase 12 antikoerper, cyclin dependent kinase 12 antikoerper, cyclin-dependent kinase 12 antikoerper, Cdk12 antikoerper, CDK12 antikoerper

Hintergrund: Name/Gene ID: CDK12
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK12, CDC2-related protein kinase 7, CRK7, Cyclin-dependent kinase 12, HCDK12, KIAA0904, CRKR, CRKRS, Pksc

Gen-ID: 51755