CXCR4 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048477

Produktdetails anti-CXCR4 Antikörper

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Maus, Ratte, Affe, Kaninchen, Hamster, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCR4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CXCR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CXCR4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (9 - 14 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Andere Bezeichnung: CXCR4 (CXCR4 Produkte)

Synonyme: CD184 antikoerper, D2S201E antikoerper, FB22 antikoerper, HM89 antikoerper, HSY3RR antikoerper, LAP3 antikoerper, LCR1 antikoerper, LESTR antikoerper, NPY3R antikoerper, NPYR antikoerper, NPYRL antikoerper, NPYY3R antikoerper, WHIM antikoerper, Cmkar4 antikoerper, PB-CKR antikoerper, PBSF/SDF-1 antikoerper, Sdf1r antikoerper, CXC-R4-B antikoerper, CXCR-4-B antikoerper, cd184 antikoerper, cxcr4 antikoerper, fb22 antikoerper, hm89 antikoerper, hsy3rr antikoerper, lap3 antikoerper, lcr1 antikoerper, lestr antikoerper, npy3r antikoerper, npyr antikoerper, npyrl antikoerper, npyy3r antikoerper, xcxcr4 antikoerper, CXC-R4 antikoerper, CXCR-4 antikoerper, d2s201e antikoerper, whim antikoerper, CXC-R4-A antikoerper, CXCR-4-A antikoerper, xCXCR4 antikoerper, CXCR4 antikoerper, cb403 antikoerper, zgc:109863 antikoerper, cb824 antikoerper, C-X-C motif chemokine receptor 4 antikoerper, chemokine (C-X-C motif) receptor 4 antikoerper, C-X-C motif chemokine receptor 4 S homeolog antikoerper, C-X-C chemokine receptor type 4 antikoerper, C-X-C motif chemokine receptor 4 L homeolog antikoerper, chemokine (C-X-C motif), receptor 4b antikoerper, chemokine (C-X-C motif) receptor 4a antikoerper, CXCR4 antikoerper, Cxcr4 antikoerper, cxcr4.S antikoerper, cxcr4 antikoerper, LOC100049444 antikoerper, cxcr4.L antikoerper, cxcr4b antikoerper, cxcr4a antikoerper

Hintergrund: Name/Gene ID: CXCR4
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR4, CD184, CD184 antigen, Cmkar4, Cxc chemokine receptor 4, CXCR-4, CXC-R4, D2S201E, FB22, Fusin, HM89, LCR1, NPY3R, NPYRL, NPYY3R, Npyy3, HSY3RR, SDF-1 receptor, WHIM, C-x-c chemokine receptor 4, LESTR, Neuropeptide Y receptor Y3, NPYR

Gen-ID: 7852

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events