Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CXCR5 Antikörper (Cytoplasmic Domain)

CXCR5 Reaktivität: Human, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048479
  • Target Alle CXCR5 Antikörper anzeigen
    CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))
    Bindungsspezifität
    • 13
    • 12
    • 9
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 92
    • 44
    • 29
    • 5
    • 5
    • 1
    • 1
    • 1
    Human, Affe
    Wirt
    • 74
    • 16
    • 13
    • 1
    Kaninchen
    Klonalität
    • 66
    • 38
    Polyklonal
    Konjugat
    • 37
    • 9
    • 9
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CXCR5 Antikörper ist unkonjugiert
    Applikation
    • 54
    • 31
    • 28
    • 10
    • 10
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human CXCR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MTA3 (50 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Dog, Bovine, Hamster, Panda, Rabbit, Pig (94%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CXCR5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Dog, Bovine, Hamster, Panda, Rabbit, Pig (94%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CXCR5 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 - 18 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))
    Andere Bezeichnung
    CXCR5 (CXCR5 Produkte)
    Synonyme
    BLR1 antikoerper, CRL1 antikoerper, CD185 antikoerper, MDR15 antikoerper, Blr1 antikoerper, CXC-R5 antikoerper, CXCR-5 antikoerper, Gpcr6 antikoerper, NLR antikoerper, C-X-C motif chemokine receptor 5 antikoerper, chemokine (C-X-C motif) receptor 5 antikoerper, CXCR5 antikoerper, Cxcr5 antikoerper
    Hintergrund
    Name/Gene ID: CXCR5
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: CXCR5, CD185 antigen, CD185, CXCR-5, CXC-R5, GPR9, MDR15, Monocyte-derived receptor 15, Blr-1, BLR1, Burkitt lymphoma receptor 1, Burkitt's lymphoma receptor 1, MDR-15
    Gen-ID
    643
Sie sind hier:
Kundenservice