CXCR7 Antikörper (Extracellular Domain)

Produktnummer ABIN1048486

Produktdetails anti-CXCR7 Antikörper

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein, Pferd, Fledermaus, Hamster, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCR7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CXCR7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Bat, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%) Marmoset, Elephant (95%) Opossum, Platypus (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 2nd extracellular domain of human CXCR7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Bat, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%), Marmoset, Elephant (95%), Opossum, Platypus (84%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Andere Bezeichnung: ACKR3 / CXCR7 (CXCR7 Produkte)

Synonyme: CMKOR1 antikoerper, CXC-R7 antikoerper, CXCR-7 antikoerper, CXCR7 antikoerper, GPR159 antikoerper, RDC-1 antikoerper, RDC1 antikoerper, AW541270 antikoerper, Cmkor1 antikoerper, Cxcr7 antikoerper, Rdc1 antikoerper, ackr3 antikoerper, cxcr7 antikoerper, rdc1 antikoerper, xcxcr7 antikoerper, atypical chemokine receptor 3 antikoerper, ORF 74; similar to Kaposi's sarcoma-associated herpesvirus ORF 74 antikoerper, atypical chemokine receptor 3 L homeolog antikoerper, ACKR3 antikoerper, MmrVgp88 antikoerper, Ackr3 antikoerper, ackr3.L antikoerper

Hintergrund: Name/Gene ID: ACKR3
Subfamily: Chemokine
Family: GPCR

Synonyms: ACKR3, Chemokine orphan receptor 1, CMKOR1, CXCR7, CXC-R7, GPRN1, RDC1, RDC-1, CXCR-7, G-protein coupled receptor 159, GPR159

Gen-ID: 57007

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling