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Dopamine Receptor d1 Antikörper (Extracellular Domain)

DRD1 Reaktivität: Human IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048514
  • Target Alle Dopamine Receptor d1 (DRD1) Antikörper anzeigen
    Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))
    Bindungsspezifität
    • 16
    • 16
    • 13
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reaktivität
    • 81
    • 64
    • 41
    • 9
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 87
    • 4
    • 1
    Kaninchen
    Klonalität
    • 79
    • 12
    Polyklonal
    Konjugat
    • 32
    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser Dopamine Receptor d1 Antikörper ist unkonjugiert
    Applikation
    • 64
    • 31
    • 27
    • 26
    • 24
    • 24
    • 16
    • 16
    • 15
    • 8
    • 8
    • 8
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human DRD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Marmoset (95%) Elephant (90%) Dog, Rabbit (85%) Horse (80%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 20 amino acid peptide from 2nd extracellular domain of human DRD1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Marmoset (95%), Elephant (90%), Dog, Rabbit (85%), Horse (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product DRD1 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (10 μg/mL)

    Not recommended for: ICC, IHC-Fr
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))
    Andere Bezeichnung
    DRD1 / Dopamine Receptor D1 (DRD1 Produkte)
    Synonyme
    DRD1 antikoerper, Dop1 antikoerper, D1A antikoerper, DADR antikoerper, DRD1A antikoerper, drd1 antikoerper, C030036C15Rik antikoerper, Drd-1 antikoerper, Drd1 antikoerper, Gpcr15 antikoerper, D1a antikoerper, Drd1a antikoerper, dopamine receptor D1 antikoerper, dopamine receptor, D1 antikoerper, dopamine receptor D1b antikoerper, DRD1 antikoerper, Dop1 antikoerper, drd1b antikoerper, Drd1 antikoerper
    Hintergrund
    Name/Gene ID: DRD1
    Subfamily: Dopamine
    Family: GPCR

    Synonyms: DRD1, D(1A) dopamine receptor, D1a dopamine receptor, Dopamine d-1 receptor, Dopamine receptor d1a, DRD1A, DADR, Dopamine d1a receptor, Dopamine receptor D1, Dopamine 1 receptor, Dopamine type 1 receptor, D-1 dopamine receptor, D1 dopamine receptor, D1a receptor, Dopamine D1 receptor
    Gen-ID
    1812
    Pathways
    cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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