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LPAR1 Antikörper (N-Term)

LPAR1 Reaktivität: Human, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048529
  • Target Alle LPAR1 Antikörper anzeigen
    LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
    Bindungsspezifität
    • 15
    • 7
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 49
    • 22
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Affe
    Wirt
    • 49
    • 3
    Kaninchen
    Klonalität
    • 47
    • 5
    Polyklonal
    Konjugat
    • 21
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser LPAR1 Antikörper ist unkonjugiert
    Applikation
    • 36
    • 18
    • 13
    • 10
    • 7
    • 7
    • 3
    • 2
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human EDG2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX60 (53 % ), ACER3 (47 %).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%) Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%) Xenopus, Pufferfish, Zebrafish (87%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 15 amino acid peptide from N-terminal extracellular domain of human EDG2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%), Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%), Xenopus, Pufferfish, Zebrafish (87%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product LPAR1 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (1 - 2 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
    Andere Bezeichnung
    LPAR1 / LPA1 / EDG2 (LPAR1 Produkte)
    Synonyme
    EDG2 antikoerper, GPR26 antikoerper, Gpcr26 antikoerper, LPA1 antikoerper, Mrec1.3 antikoerper, VZG1 antikoerper, edg-2 antikoerper, rec.1.3 antikoerper, vzg-1 antikoerper, Edg2 antikoerper, EDG-2 antikoerper, 5031439C20 antikoerper, AI326300 antikoerper, Kdt2 antikoerper, lpA1 antikoerper, edg2 antikoerper, si:dz22b13.1 antikoerper, si:dz46m7.1 antikoerper, zgc:92157 antikoerper, LPA receptor 1-A antikoerper, LPA-1-A antikoerper, gpr26 antikoerper, lpa1 antikoerper, lpa1r antikoerper, lpa1r1 antikoerper, lpar1 antikoerper, lpar1-a antikoerper, lpar1-b antikoerper, vzg1 antikoerper, xLPA1-1 antikoerper, LPA receptor 1-B antikoerper, LPA-1-B antikoerper, edg2-A antikoerper, xLPA1-2 antikoerper, xlpa1R antikoerper, lysophosphatidic acid receptor 1 antikoerper, lysophosphatidic acid receptor 1 S homeolog antikoerper, lysophosphatidic acid receptor 1 L homeolog antikoerper, LPAR1 antikoerper, Lpar1 antikoerper, lpar1 antikoerper, lpar1.S antikoerper, lpar1.L antikoerper
    Hintergrund
    Name/Gene ID: LPAR1
    Subfamily: Lysophospholipid/Lysosphingolipid
    Family: GPCR

    Synonyms: LPAR1, EDG-2, EDG2, Gpcr26, Rec.1.3, Vzg-1, Ventricular zone gene 1, LPA receptor 1, LPA-1, LPA1, Mrec1.3, VZG1
    Gen-ID
    1902
    Pathways
    Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration
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