LPAR1 Antikörper (N-Term)
-
- Target Alle LPAR1 Antikörper anzeigen
- LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
-
Bindungsspezifität
- N-Term
-
Reaktivität
- Human, Affe
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser LPAR1 Antikörper ist unkonjugiert
-
Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human EDG2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX60 (53 % ), ACER3 (47 %).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%) Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%) Xenopus, Pufferfish, Zebrafish (87%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
-
Synthetic 15 amino acid peptide from N-terminal extracellular domain of human EDG2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%), Mouse, Rat, Sheep, Dog, Bovine, Bat, Hamster, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (93%), Xenopus, Pufferfish, Zebrafish (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LPAR1 Primärantikörper
-
-
- Applikationshinweise
- Approved: IHC, IHC-P (1 - 2 μg/mL)
- Kommentare
-
Target Species of Antibody: Human
- Testdurchführung
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
- Andere Bezeichnung
- LPAR1 / LPA1 / EDG2 (LPAR1 Produkte)
- Synonyme
- EDG2 antikoerper, GPR26 antikoerper, Gpcr26 antikoerper, LPA1 antikoerper, Mrec1.3 antikoerper, VZG1 antikoerper, edg-2 antikoerper, rec.1.3 antikoerper, vzg-1 antikoerper, Edg2 antikoerper, EDG-2 antikoerper, 5031439C20 antikoerper, AI326300 antikoerper, Kdt2 antikoerper, lpA1 antikoerper, edg2 antikoerper, si:dz22b13.1 antikoerper, si:dz46m7.1 antikoerper, zgc:92157 antikoerper, LPA receptor 1-A antikoerper, LPA-1-A antikoerper, gpr26 antikoerper, lpa1 antikoerper, lpa1r antikoerper, lpa1r1 antikoerper, lpar1 antikoerper, lpar1-a antikoerper, lpar1-b antikoerper, vzg1 antikoerper, xLPA1-1 antikoerper, LPA receptor 1-B antikoerper, LPA-1-B antikoerper, edg2-A antikoerper, xLPA1-2 antikoerper, xlpa1R antikoerper, lysophosphatidic acid receptor 1 antikoerper, lysophosphatidic acid receptor 1 S homeolog antikoerper, lysophosphatidic acid receptor 1 L homeolog antikoerper, LPAR1 antikoerper, Lpar1 antikoerper, lpar1 antikoerper, lpar1.S antikoerper, lpar1.L antikoerper
- Hintergrund
-
Name/Gene ID: LPAR1
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR
Synonyms: LPAR1, EDG-2, EDG2, Gpcr26, Rec.1.3, Vzg-1, Ventricular zone gene 1, LPA receptor 1, LPA-1, LPA1, Mrec1.3, VZG1 - Gen-ID
- 1902
- Pathways
- Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration
-