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ERAP1 Antikörper (Internal Region)

ERAP1 Reaktivität: Human, Hamster IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1048575
  • Target Alle ERAP1 Antikörper anzeigen
    ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))
    Bindungsspezifität
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 32
    • 17
    • 15
    • 1
    Human, Hamster
    Wirt
    • 30
    • 2
    Kaninchen
    Klonalität
    • 28
    • 4
    Polyklonal
    Konjugat
    • 21
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ERAP1 Antikörper ist unkonjugiert
    Applikation
    • 19
    • 15
    • 10
    • 9
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human ERAP1 / ARTS1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Hamster (100%) Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig (94%) Mouse, Rat, Bat, Rabbit, Opossum, Guinea pig, Platypus (88%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from internal region of human ERAP1 / ARTS1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Hamster (100%), Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig (94%), Mouse, Rat, Bat, Rabbit, Opossum, Guinea pig, Platypus (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product ERAP1 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (10 - 15 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))
    Andere Bezeichnung
    ARTS1 / ERAP1 (ERAP1 Produkte)
    Synonyme
    MGC166160 antikoerper, DKFZp469C044 antikoerper, ARTS-1 antikoerper, A-LAP antikoerper, ALAP antikoerper, APPILS antikoerper, ARTS1 antikoerper, ERAAP antikoerper, ERAAP1 antikoerper, PILS-AP antikoerper, PILSAP antikoerper, Arts1 antikoerper, PILSA antikoerper, Appils antikoerper, endoplasmic reticulum aminopeptidase 1 antikoerper, endoplasmic reticulum aminopeptidase 1b antikoerper, ERAP1 antikoerper, erap1 antikoerper, erap1b antikoerper, Erap1 antikoerper
    Hintergrund
    Name/Gene ID: ERAP1
    Subfamily: Metallopeptidase M1
    Family: Exopeptidase

    Synonyms: ERAP1, A-LAP, ALAP, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILS-AP, Aminopeptidase PILS, ARTS-1, PILSAP
    Gen-ID
    51752
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