ERAP1 Antikörper (Internal Region)

Produktnummer ABIN1048575

Produktdetails anti-ERAP1 Antikörper

Target: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERAP1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ERAP1 / ARTS1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Hamster (100%) Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig (94%) Mouse, Rat, Bat, Rabbit, Opossum, Guinea pig, Platypus (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from internal region of human ERAP1 / ARTS1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Hamster (100%), Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig (94%), Mouse, Rat, Bat, Rabbit, Opossum, Guinea pig, Platypus (88%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 - 15 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ERAP1

Target: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Andere Bezeichnung: ARTS1 / ERAP1 (ERAP1 Produkte)

Synonyme: MGC166160 antikoerper, DKFZp469C044 antikoerper, ARTS-1 antikoerper, A-LAP antikoerper, ALAP antikoerper, APPILS antikoerper, ARTS1 antikoerper, ERAAP antikoerper, ERAAP1 antikoerper, PILS-AP antikoerper, PILSAP antikoerper, Arts1 antikoerper, PILSA antikoerper, Appils antikoerper, endoplasmic reticulum aminopeptidase 1 antikoerper, endoplasmic reticulum aminopeptidase 1b antikoerper, ERAP1 antikoerper, erap1 antikoerper, erap1b antikoerper, Erap1 antikoerper

Hintergrund: Name/Gene ID: ERAP1
Subfamily: Metallopeptidase M1
Family: Exopeptidase

Synonyms: ERAP1, A-LAP, ALAP, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILS-AP, Aminopeptidase PILS, ARTS-1, PILSAP

Gen-ID: 51752