ESRRG Antikörper (C-Term)
-
- Target Alle ESRRG Antikörper anzeigen
- ESRRG (Estrogen-Related Receptor gamma (ESRRG))
-
Bindungsspezifität
- C-Term
-
Reaktivität
- Human, Maus, Ratte, Hund, Kaninchen, Rind (Kuh), Pferd, Affe, Huhn, Schwein, Hamster
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser ESRRG Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)
- Marke
- IHC-plus™
- Spezifität
- Human ESRRG / ERR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%) Bat, Xenopus, Zebrafish (93%) Stickleback, Medaka, Pufferfish (87%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
-
Synthetic 15 amino acid peptide from C-terminus of human ESRRG / ERR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%), Bat, Xenopus, Zebrafish (93%), Stickleback, Medaka, Pufferfish (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ESRRG Primärantikörper
-
-
- Applikationshinweise
- Approved: ChrIP, IHC, IHC-P (9 - 17 μg/mL), WB
- Kommentare
-
Target Species of Antibody: Human
- Testdurchführung
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- ESRRG (Estrogen-Related Receptor gamma (ESRRG))
- Andere Bezeichnung
- ESRRG / ERR gamma (ESRRG Produkte)
- Synonyme
- ERR3 antikoerper, ERRgamma antikoerper, NR3B3 antikoerper, ESRRG antikoerper, USH2A antikoerper, DKFZp459J0417 antikoerper, err3 antikoerper, nr3b3 antikoerper, LOC100219115 antikoerper, errg antikoerper, errgamma antikoerper, esrrg antikoerper, errb/g antikoerper, esrrgl antikoerper, errbeta/gamma antikoerper, Errg antikoerper, mKIAA0832 antikoerper, estrogen related receptor gamma antikoerper, estrogen-related receptor gamma antikoerper, estrogen-related receptor gamma a antikoerper, estrogen-related receptor gamma b antikoerper, ESRRG antikoerper, esrrg antikoerper, Esrrg antikoerper, esrrga antikoerper, esrrgb antikoerper
- Hintergrund
-
Name/Gene ID: ESRRG
Subfamily: NR3 Steroid receptor
Family: NHR
Synonyms: ESRRG, ERR Gamma, ERR gamma-2, ERR3, Err-gamma, NR3B3, ERRG2, ERRgamma, KIAA0832 - Gen-ID
- 2104
- Pathways
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
-